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Immunogenicity and vaccine approach of Salmonella enterica sorovar Typhimurium flagellins.

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Author(s):
Liliana Moura Massis
Total Authors: 1
Document type: Doctoral Thesis
Press: São Paulo.
Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
Defense date:
Examining board members:
Luis Carlos de Souza Ferreira; Marcelo Brocchi; Marilis do Valle Marques; Maria Leonor Sarno de Oliveira; Carlos Pelleschi Taborda
Advisor: Luis Carlos de Souza Ferreira; Maria Elisabete Sbrogio de Almeida
Abstract

Flagellin, the structural subunit of bacterial flagella, can be used in vaccine development as a fused antigenic epitope carrier or as an adjuvant. In this work we evaluated the immunogenicity and vaccine approach of S. Typhimurium flagellin in BALB/c mice after administration by oral or intraperitoneal route. Our results indicate that mice immunized by oral route with flagellated S. Typhimurium strains do not develop any anti-flagellin antibody response, be it serum or secreted. On the other hand, specific anti-flagellin cellular response was observed depending on the chosen route of immunization with S. Typhimurium attenuated strain. Furthermore, all flagellins tested proved to be efficient adjuvants when co-administrated with CFA/I fimbriae by nasal route. Together, these results contribute to a better understanding of the immunological and adjuvant properties of S. Typhimurium flagellins and also provide information on the rational use of these proteins in vaccine development. (AU)