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| Author(s): |
Arianne Costa Baquião
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2012-04-18 |
| Examining board members: |
Benedito Correa;
Andrezza Maria Fernandes;
Marcelo Elias Fraga;
Carlos Augusto Fernandes de Oliveira;
Myrna Sabino
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| Advisor: | Benedito Correa |
| Abstract | |
The objective of this present study was analyse, in the field and in the storage, mycobiota and the contamination by (aflatoxins and cyclopiazonic acid from Brazil nuts, soil and air samples to determine route of fungi contamination. The Brazil nuts mycobiota was determine by diluition plating method in Potate Dextrose Agar and Aspergillus flavus parasiticus agar and, micotoxins was done by high performance liquid chromatography. Field samples were collected in: day 0, samples still on the tree; days 5, 10 and 15, samples in contact with soil for 5, 10 and 15 days, respectively. The most prevalent fungi were Aspergillus flavus in fruit pods and nuts and Fusarium spp. in shells. Penicillium spp. and A. flavus were isolated from soil, and Fusarium spp. and Penicillium spp. from air. The storage samples were analysed montly during 11 months; and showed predominance A. flavus, Fusarium spp. e A. nomius in nuts, and Fusarium spp. and A. flavus in shells. Aflatoxins and cyclopiazonic acid were not detected. These findings indicate soil as the main source of fungal contamination of Brazil nuts. A. nomius e A. parasiticus were isolated only in storage, suggesting Brazil nuts contamination occurs in this period. (AU) | |