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| Author(s): |
Rominne Karla Barros Freire
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2012-06-05 |
| Examining board members: |
Luiziana Ferreira da Silva;
Rodrigo da Silva Galhardo;
Jose Geraldo da Cruz Pradella
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| Advisor: | Luiziana Ferreira da Silva |
| Abstract | |
Lignocellulosic residues are remarkable substrates for the sustainable production of polyhydroxyalkanoates (PHAs) and ethanol. Xylose is one of the most important lignocelullose component but its efficient utilization still represents a technical barrier. The aim of this work was to obtain bacterial strains more efficient in the xylose consumption. Multiple copies of the catabolism (xylAB) and transport (xylFGH) genes of xylose were introduced in the ethanol producer Escherichia coli KO11 strain and the poly-3-hydroxybutyrate (PHB) producer Burkholderia sacchari LFM 101. The recombinants strains were evaluated for their production and xylose consumption in the presence and absence of glucose. This strategy did not increase xylose consumption in B. sacchari strains. The xylAB gene improved xylose consumption and increased the ethanol production about 30% in E. coli KO11, but this effect was impaired by catabolite repression; while xylFGH gene was deleterious to reduce the growth and ethanol production by this strain. (AU) | |
| FAPESP's process: | 09/11933-2 - Construction of recombinant bacteria to produce ethanol and biopolymers from sugars present on sugarcane bagasse hydrolysates |
| Grantee: | Rominne Karla Barros Freire |
| Support Opportunities: | Scholarships in Brazil - Master |