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| Author(s): |
Atila Iamarino
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2012-08-14 |
| Examining board members: |
Paolo Marinho de Andrade Zanotto;
Gonzalo Jose Bello Bentancor;
Maria Inês de Moura Campos Pardini;
Enrique Mario Boccardo Pierulivo;
Tiago José Paschoal Sobreira
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| Advisor: | Paolo Marinho de Andrade Zanotto |
| Abstract | |
Despite general efforts to control HIV-1 infections, many recombinants have been described among B and F subtype in South America. During the VGDN HIV task in São Paulo state, a large number of new BF recombinants was found, most of which harbor a subtype F protease. Phylogenetic analysis of integrase genes from these patients showed distinct origins for similar recombinant regions. Phylodynamic analysis suggested that after HAART introduction in Argentina BF recombinants with F protease depicted a sustained growth, while subtype B had diminished in growth.. Moreover, structural data from proteases after HAART introduction, while subtype B had diminished growth. Subtype F proteases structure and nelfinavir interaction measured by molecular dynamics suggested that polymorphisms of this subtype may restore enzyme activity or decrease even further inhibitor affinity. A pNL4-3 recombinant vector with most of gag gene and a protease of subtype F was made, which allows the comparison of its effects on viral fitness. (AU) | |