Production and characterization of a monoclonal antibody aDEC205 coupled to MSP-1(19) protein from Plasmodium chabaudi.

Author(s)

Raquel Hoffmann Panatieri

• Document type: Master's Dissertation
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2011-05-13

Examining board members

Maria Regina D'Imperio Lima; Fábio Trindade Maranhão Costa; Claudio Romero Farias Marinho

• Advisor: Maria Regina D'Imperio Lima

Abstract

Despite the strong activation of the immune system that occurs during infection by Plasmodium, the immunological memory to infection is restricted to patients residing in endemic areas. Thus it is important to the generation of methods to induce an effective immune response against the parasite. In this context, the targeting of antigens to the central cells of the immune system has emerged as a promising alternative. We produce and characterize a hybrid antibody molecule specific for DEC205, an endocytic receptor present on dendritic cells, coupled to protein MSP-1(19) of P. chabaudi, for immunization and analysis of cellular and humoral immune response. Immunization tests showed the induction of humoral response in mice immunized with hybrid antibody and isotype control, characterized by production of IgM. Our previous results indicate that targeting antigens combined with other strategies for immunization may result in the activation of specific immune response to the parasite. (AU)