Synthesis of complement proteins by monocyte-derived dendritic cells developed in the presence of tumor supernatants.

Author(s)

Giovana Cechim

• Document type: Master's Dissertation
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2012-02-03

Examining board members

Jose Alexandre Marzagao Barbuto; Ana Paula Lepique; Ana Paula Duarte de Souza

• Advisor: Jose Alexandre Marzagao Barbuto

Abstract

Antigen presentation by dendritic cells (DC) to T cells is the first step to generate an antitumor immune response. Tumors interfere in this process, affecting DCs function. One of the factors that affect DCs´s function is the complement system protein C3, which is produced by these cells. In this work, we investigated the influence of tumor supernatants derived from human glioma cells lines U87MG and A172 in the production of complement protein C3 by monocyte-derived dendritic cells from healthy donors in vitro. DC phenotyping indicated that the supernatants seem to modulate the expression of CD14,CD80,CD86, CD83, CD274 and CD11b. The expression of C3 gene, was negatively modulated by U87MG supernatant while the A172 supernatant seemed to exert the reverse effect. (AU)

• FAPESP's process: 10/13803-6 - SYNTHESIS OF COMPLEMENT PROTEINS BY MONOCYTE-DERIVEDDENDRITIC CELLS DEVELOPED IN THE PRESENCE OF TUMOR SUPERNATANTS
• Grantee: Giovana Cechim
• Support Opportunities: Scholarships in Brazil - Master