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| Author(s): |
Maíra Estanislau Soares de Almeida
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2011-08-10 |
| Examining board members: |
Marinilce Fagundes dos Santos;
Ruy Gastaldoni Jaeger;
Marimelia Aparecida Porcionatto
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| Advisor: | Marinilce Fagundes dos Santos |
| Abstract | |
Diabetes mellitus (DM) is characterized by chronic hyperglycemia (HG), which causes several complications, including impaired wound healing. We investigated the effects of HG on the migration of primary dermal fibroblasts on collagen and fibronectin, using two-dimensional (2-D) and three-dimensional (3-D) substrates. We observed that HG reduced migration velocity on both matrices. Systemic treatment with antioxidants prevented these effects. The velocity of protrusion formation was unaffected, but a decrease in protrusion stability was observed. HG slightly interfered with cell adhesion on collagen and fibronectin, but cell spreading was reduced on collagen and increased on fibronectin. Accordingly, the distribution of the integrin subunits <font face=\"Symbol\">a1, <font face=\"Symbol\">av and <font face=\"Symbol\">a5 was affected by HG. This study shows that the effects of HG on cell migration involve basic mechanisms common to various substrates, as well as mechanisms specifically related to fibronectin, possibly involving oxidative stress and adhesion signaling. (AU) | |
| FAPESP's process: | 09/06658-2 - Hyperglycemia and cell-extracellular matrix interactions: effects on fibroblasts migration and implications for the healing of chronic wounds in diabetic patients |
| Grantee: | Maíra Estanislau Soares de Almeida |
| Support Opportunities: | Scholarships in Brazil - Master |