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| Author(s): |
Tatiane Yumi Nakamura Kanno
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2011-10-14 |
| Examining board members: |
Chao Yun Irene Yan;
Enilza Maria Espreafico;
Ruy Gastaldoni Jaeger
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| Advisor: | Chao Yun Irene Yan |
| Abstract | |
The dorsal root ganglia (DRG) harbor the TrkA-positive neurons. The stimuli perception and transmission by these neurons depend on a proper neuritogenesis. Myosin (MyoVa) is widely expressed in nervous tissue and is present in neurites, cell body and growth cone. Here, we characterized the MyoVa expression pattern in chicken DRG TrkA-positive cells neuritogenesis, in vivo and in vitro. In vivo, at stage HH25, MyoVa was present both in cells with and without neurites and its expression persists throughout neuritogenesis. In vitro, it is recruited for the regeneration process and TrkA-positive neurons neurites re-emission in the presence of NGF, being expressed in neurites at different stages of neo-neuritogenesis. In functional assays, we observed that MyoVa globular tail overexpression in GRD cultures maintained with 10 or 100ng/ml NGF reduces the number of neurons with long neurites and increased the number of neurons with short neurites or no neurites. Taken together, these results suggest that Myosin Va is important for the establishment of nociceptor neurites. (AU) | |
| FAPESP's process: | 09/03132-0 - The role of myosin VA in dorsal root ganglia neuritogenesis |
| Grantee: | Tatiane Yumi Nakamura Kanno |
| Support Opportunities: | Scholarships in Brazil - Master |