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| Author(s): |
Hugo Rezende Henriques
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2012-03-28 |
| Examining board members: |
Silvia Beatriz Boscardin;
Juliano Bordignon;
Daniela Santoro Rosa
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| Advisor: | Silvia Beatriz Boscardin |
| Abstract | |
Dendritic Cells are critical in the interaction between innate and the adaptive immune system, presenting antigens to T lymphocytes and inducing adaptive immune responses against these. DCs are target for development of new vaccine strategies based on targeting antigens to different DCs populations in vivo. This consists on the administration of antibodies specific to DC surface endocytic receptor fused to an antigen. In this study, we evaluated the differences in adaptive responses when the NS1 protein from the DENV2 was directed to the two main populations of DCs in the spleen along with different agonistic molecules. Highest CD8+ T cell responses were seen when Poly I:C was used, but only when directed to the CD8+DEC205+ DC population. In the presence of agonist CpG we observed similar responses when the protein was targeted to either CD8+DEC205+ or CD8-DCIR2+ DCs. In the presence of Poly I:C, only targeting to the DEC205+ DCs was able to protect mice against a challenge with DENV2. This protection was dependent on both CD8+ and CD4+ T cell responses. (AU) | |
| FAPESP's process: | 10/03079-9 - Adaptive Response analysis when a dengue virus antigen is directed to two distinct populations of dendritic cells. |
| Grantee: | Hugo Rezende Henriques |
| Support Opportunities: | Scholarships in Brazil - Master |