Evaluation of humoral and cellular immune responses induced by targeting the domain III of the dengue virus envelope protein to the DEC205+ dendritic cell subpopulation.

• Grant number: 15/16565-2
• Support Opportunities: Scholarships in Brazil - Master
• Start date: February 01, 2016
• End date: August 31, 2017
• Field of knowledge: Biological Sciences - Immunology - Applied Immunology
• Principal Investigator: Silvia Beatriz Boscardin
• Grantee: Bianca da Silva Almeida
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Dendritic cells (DCs) are considered the best antigen presenting cells because they have the ability to acquire different types of pathogens and present their antigens efficiently to T lymphocytes. The Laboratory of Antigen Targeting to Dendritic Cells (ICB-USP) is developing successfully strategies aimed at targeting antigens to DCs in vivo in mice. This strategy is based on the use of a monoclonal antibody (mAb) produced specifically to react with receptors present on the surface of DCs fused to an antigen of interest. One of these receptors is called DEC205 (or CD205). The administration of low doses of chimeric anti-DEC205 mAbs fused proteins derived from different pathogens induces strong T-cell responses and antibody production against the protein used. Dengue is a viral disease that affects millions of people worldwide and various vaccine strategies based on the proteins that make up the viral particles are being studied. Among these proteins, there is the virus envelope (E) protein. The E protein structure can be divided into three domains (I, II and III) which have different functions in the adhesion and invasion of the target cell. Domain III (EDIII) is of particular interest because it appears to contain epitopes against which neutralizing antibodies have been obtained. This research project aims to evaluate in more detail the humoral and cellular immune responses induced after immunization of animals with chimeric anti-DEC205 mAbs fused to the domain III of protein E.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SULCZEWSKI, FERNANDO BANDEIRA; MARTINO, LARISSA ALVES; ALMEIDA, BIANCA DA SILVA; ZANETI, ARTHUR BARUEL; FERREIRA, NATALIA SOARES; DA SILVA AMORIM, KELLY NAZARE; YAMAMOTO, MARCIO MASSAO; APOSTOLICO, JULIANA DE SOUZA; ROSA, DANIELA SANTORO; BOSCARDIN, SILVIA BEATRIZ. Conventional type 1 dendritic cells induce T(H)1, T(H)1-like follicular helper T cells and regulatory T cells after antigen boost via DEC205 receptor. European Journal of Immunology, v. 50, n. 12, JUL 2020. (18/20821-2, 15/18874-2, 17/06503-5, 15/16565-2, 18/00145-2, 18/07142-9)

ZANETI, ARTHUR BARUEL; YAMAMOTO, MARCIO MASSAO; SULCZEWSKI, FERNANDO BANDEIRA; ALMEIDA, BIANCA DA SILVA; SANTOS SOUZA, HIGO FERNANDO; FERREIRA, NATALIA SOARES; NASCIMENTO FABRIS MAEDA, DENICAR LINA; SALES, NATIELY SILVA; ROSA, DANIELA SANTORO; DE SOUZA FERREIRA, LUIS CARLOS; et al. Dendritic Cell Targeting Using a DNA Vaccine Induces Specific Antibodies and CD4(+) T Cells to the Dengue Virus Envelope Protein Domain III. FRONTIERS IN IMMUNOLOGY, v. 10, JAN 29 2019. (16/04477-4, 13/11442-4, 14/50631-0, 14/17595-0, 15/16565-2, 14/15061-8, 15/18874-2)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

ALMEIDA, Bianca da Silva. Analysis of the cellular and humoral immune responses induced after targeting of the dengue virus envelope protein to the DEC205+ dendritic cells.. 2017. Master's Dissertation - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.