Effect of short chain fatty acids on neutrophils function.

Author(s)

Marco Aurelio Ramirez Vinolo

• Document type: Doctoral Thesis
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2010-11-30

Examining board members

Rui Curi; Fernando Rodrigues de Moraes Abdulkader; Niels Olsen Saraiva Câmara; Primavera Borelli Garcia; Francisco Garcia Soriano

• Advisor: Rui Curi

Abstract

We evaluated the effect of SCFA (acetate, propionate and butyrate) on the recruitment of neutrophils and functional parameters (reactive oxygen species [ROS], cytokines and nitric oxide production, phagocytosis and destruction of C. albicans). We also investigated the activation of NFkB, effect on histone deacetylases (HDAC) and GPR43. Acetate and butyrate altered the production of ROS, the former increased the production of hydrogen peroxide, whereas butyrate inhibited the production stimulated by PMA. Butyrate reduced the phagocytosis and killing of yeast. Propionate and butyrate reduced the production of TNF-<font face=\"Symbol\">&#945, CINC-2<font face=\"Symbol\">&#945<font face=\"Symbol\">b and nitric oxide and increased the synthesis of IL-1<font face=\"Symbol\">&#946 by LPS-stimulated neutrophils. These effects involve modification at the transcriptional level and inhibition of HDAC and NFkB activation. SCFA increased neutrophil migration in vitro and in vivo, an effect that may be the result of increased production of CINC-2<font face=\"Symbol\">&#945<font face=\"Symbol\">b and direct action of GPR43. SCFA present pro- and anti-inflammatory actions depending on the parameter analyzed. (AU)

• FAPESP's process: 06/00372-1 - Role of GPR43 on macrophage function: possible mechanism of action of short-chain fatty acids
• Grantee: Marco Aurélio Ramirez Vinolo
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)