Effects of treatment with oleic or linoleic acids in vitro or in vivo on the production of inflammatory mediators by macrophages.

Author(s)

Juliana Magdalon

• Document type: Master's Dissertation
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2011-08-15

Examining board members

Rui Curi; Thais Martins de Lima Salgado; Sandra Coccuzzo Sampaio Vessoni

• Advisor: Rui Curi

Abstract

The aim of this study was to investigate the effects of treatment with oleic or linoleic acids on the production of inflammatory mediators by macrophages, comparing in vivo and in vitro treatments. Resident macrophages were used and harvested by rat peritoneal lavage. For experiments using in vitro treatment, cells were treated with 25 <font face=\"Symbol\">mM, 50 <font face=\"Symbol\">mM and 100 <font face=\"Symbol\">mM of fatty acids. For those using in vivo treatment, animals were supplemented for 10 days with 0.15 g/kg body weight of water (control), pure oleic or linoleic acids. The fatty acids were not toxic at the doses used, according to the measurement of fragmented DNA and MTT assay. Overall, in vitro treatment with oleic or linoleic acids decreased ROS, nitrite and TNF-<font face=\"Symbol\">a production and increased IL-6, CINC-2<font face=\"Symbol\">a<font face=\"Symbol\">b and VEGF production. In vivo treatment with the same fatty acids decreased TNF-<font face=\"Symbol\">a, IL-1<font face=\"Symbol\">b, IL-6, CINC-2<font face=\"Symbol\">a<font face=\"Symbol\">b and VEGF production. Therefore, the present study suggests that oleic and linoleic acids may be used as therapeutic agents. (AU)

• FAPESP's process: 09/03488-9 - Effect of oleic, linoleic and palmitic acids on cellular proliferation and alpha-enolase and FBP (Far upstream element-binding protein)expression in murine fibroblasts: possible application on healing process
• Grantee: Juliana Magdalon
• Support Opportunities: Scholarships in Brazil - Master