Modulation of the wound healing by oleci and linoleic acids supplementation.

Author(s)

Hosana Gomes Rodrigues

• Document type: Doctoral Thesis
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2011-08-26

Examining board members

Rui Curi; Maria Fernanda Cury Boaventura; Lydia Masako Ferreira; Marinilce Fagundes dos Santos; Francisco Garcia Soriano

• Advisor: Rui Curi

Abstract

This study aimed to characterize the healing process and to investigate the effects of supplementation (gavage) with OL and Li on the healing process through in vivo experiments. After anesthesia, an area of 10 mm2 of skin was surgically removed from the dorsum. Li reduced the size of the wounds on the seventh day after induction. This result was accompanied by increased production of hydrogen peroxide (H2O2). OL reduced the H2O2 content after 24 hours. Li accelerated the migratory response, since inflammatory cells were found one hour after the wound. OL activated the transcription factor NF-<font face=\"Symbol\">kB and Li increased the activation of AP-1 one hour after wound induction. Both fatty acids reduced the activation of NF-<font face=\"Symbol\">kB and did not alter AP-1 after 24 hours. Li raised the concentrations of CINC-2<font face=\"Symbol\">a<font face=\"Symbol\">b and OL increased TNF-<font face=\"Symbol\">a, in the homogenate, one hour after wound induction. In 24 hours, the FA reduced expression and concentrations of IL-1<font face=\"Symbol\">b, IL-6 and MIP-3<font face=\"Symbol\">a. Putting together, our results demonstrate that OL and Li accelerate the inflammatory phase of the wound healing. (AU)