Full text
| |
| Author(s): |
Carlos Hermano da Justa Pinheiro
Total Authors: 1
|
| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2012-06-26 |
| Examining board members: |
Rui Curi;
Niels Olsen Saraiva Câmara;
Celso Ricardo Fernandes de Carvalho;
Maria Tereza Nunes;
Leonardo dos Reis Silveira
|
| Advisor: | Rui Curi |
| Abstract | |
The purpose of the present study was to investigate alterations on fatty acid (FA) composition and a possible involvement of FA on muscle stem cell function. Na increase in content of oleic acid was observed when muscle stem cell (muscSC) differentiated to myotube. The treatment with oleic acid accelerated myogenesis through stimulation of beta-catenin signaling pathway. Notch signaling pathway was inhibited by oleic acid reducing the proliferative capacity of muscSC. No effects were observed in mesenchymal stem cells. Transplantation of both muscSC and mesenchymal stem cells accelerates muscle regeneration and recovery of function. The pre-treatment with oleic acid decreased activation, proliferation, differentiation and engrafment of muscSC in host injured muscle. Thus, oleic acid has modulatory effect on cellular therapy in injured skeletal muscle. (AU) | |