The effect of hypertension and diabetes association on Slc2a4/GLUT4 expression, possible b-adrenergic sympathectic nervous system participation.

Author(s)

Ana Bárbara Teixeira Alves Wagner

• Document type: Doctoral Thesis
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2012-09-14

Examining board members

Ubiratan Fabres Machado; Marcelo Augusto Christoffolete; William Tadeu Lara Festuccia; Gisele Giannocco; Márcia Silva Queiroz

• Advisor: Ubiratan Fabres Machado

Abstract

We investigated the participation of sympathetic activity in the regulation of Slc2a4/GLUT4 expression in skeletal muscle of diabetic Wistar and spontaneously hypertensive (SHR) rats, treated with saline, insulin, propranolol and propranolol+insulin. Insulin reduced glycemia, urinary volume and glucose, and increased body weight and Slc2a4 expression in soleus, but decreased it in EDL. Propranolol reduced arterial blood pressure in SHR, and did not alter the metabolic parameters, increased the mRNA expression in EDL, but reduced it in soleus of normotensive rats. Propranolol+insulin showed that in soleus the effect of insulin was preponderant, whereas in EDL propranolol attenuated the insulin effect. The Slc2a4 expression was higher in SHR (soleus, EDL), and it was similar to the protein expression, with some discrepancies, indicating post-transcription regulation, or Tnfa-related alteration. In conclusion, the impairment in Slc2a4 expression during diabetes is limited in SHR, probably because their high sympathetic activity in skeletal muscle. (AU)