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| Author(s): |
Stelamar Romminger
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Carlos. |
| Institution: | Universidade de São Paulo (USP). Instituto de Química de São Carlos (IQSC/BT) |
| Defense date: | 2008-11-20 |
| Examining board members: |
Roberto Gomes de Souza Berlinck;
Angela Regina Araujo;
Hosana Maria Debonsi
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| Advisor: | Roberto Gomes de Souza Berlinck |
| Abstract | |
Fungi are widely disperse microorganisms, typically associated with plants, animals, soil and aquatic environments (fresh and sea water), participating in the elements cycling. Although many ecological roles have been described for terrestrial fungi, ecological studies of marine derived fungi are still scarce. Therefore, oceans, which represent approximately half of the global biodiversity, are a huge and virtually unexplored source of microorganisms producers of interesting metabolites. The aim of this study was to isolate fungal strains derived from a marine algae of the Sargassum genus, and the evaluation of their metabolical potential for the production of secondary metabolites. Overall, 58 strains were isolated, of which 52 were grown in liquid culture media and extracted with organic solvents, originating 99 crude extracts. These extracts were analyzed by bioassays, thin layer chromatography (TLC), nuclear magnetic resonance (NMR) and liquid chromatography coupled with a photo diode array, an evaporative light scattering, and a mass spectrometry detectors (LC - PDA - ELSD - MS). The evaluation with the antibiotic assay resulted in the largest number of active extracts (n = 13), followed by the enzymatic (n = 8), the cytotoxic (n = 3) and the antituberculosis (n = 1) assays. The crude extract AS Fub 39, which presented antibiotic activity, was selected for additional studies. This extract was purified by HPLC, and its major compound identified as the 8-methoxy-3,5-dimethylisocroman-6-ol. Later, the AS Fub 39 strain was taxonomically identified as Penicillium steckii. (AU) | |
| FAPESP's process: | 06/58840-0 - Evaluation of the metabolic potential of fungal lineages isolated from marine algae of the genus Sargassum sp. |
| Grantee: | Stelamar Romminger |
| Support Opportunities: | Scholarships in Brazil - Master |