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| Author(s): |
Katia Nardelli Greco
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2009-10-16 |
| Examining board members: |
Ronaldo Zucatelli Mendonça;
José Antonio Jerez;
Alvaro Paiva Braga de Sousa
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| Advisor: | Ronaldo Zucatelli Mendonça |
| Abstract | |
Several works have demonstrated the presence of bioactive peptides in insect hemolymph and their potential use as therapeutic agents. This work sought to identify and purify proteins from Lonomia obliqua hemolymph with antiviral activity. The analyses demonstrated that the best time for hemolymph addition in the cell culture is before the infection, the poliovirus titer had reduced from 1.5x107 TCID50/mL to 4x105 TCID50/mL and for measles, the virus titer was about 100 times lower than the control. After gel filtration chromatography, the hemolymph was divided into 3 pools of protein. The pool responsible for the antiviral activity was identified (Pool 2), once it reduced to 87 times the poliovirus titer, and the measles titer from 1.6x106 TCID50/mL to 2.7x105 TCID50/mL. The Pool 2 was fractionated by ion-exchange chromatography. The fraction from Lonomia obliqua hemolymph responsible for the antiviral activity was identified - RQ 3-4, this protein of approximately 20 kDa, has antiviral effect on enveloped virus (measles) and on non enveloped virus (poliovirus). (AU) | |