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Diversity of archaea and bacteria involved in the nitrogen cycling in mangrove sediments

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Author(s):
Armando Cavalcante Franco Dias
Total Authors: 1
Document type: Doctoral Thesis
Press: Piracicaba.
Institution: Universidade de São Paulo (USP). Centro de Energia Nuclear na Agricultura (CENA/STB)
Defense date:
Examining board members:
João Lucio de Azevedo; Tsai Siu Mui; Valéria Maia de Oliveira; Vivian Helena Pellizari
Advisor: João Lucio de Azevedo; Fernando Dini Andreote
Abstract

The mangrove is a coastal ecosystem, located in the interface regions between the land and sea environments, with exclusive occurrence in tropical and subtropical areas. Such location confers to the mangrove sediments unique characteristics, like as high salinity and low availability of oxygen, associated with the high abundance of organic matter. The result of these conditions is the occurrence of a restrict plant diversity, associated with a great diversity of animals, who use the mangroves for its protection and reproduction. The present study has shown how the communities of archaea (16S DNAr and amoA) and bacteria (16S DNAr and nifH) are structured in mangrove soils under distinct states of preservation. The DGGE patterns indicate alterations in the composition of targeted communities, linking its structure with the environmental contamination, while the quantifications of targeted genes by real time PCR (qPCR) has indicated alterations only in the community of ammonium oxidizers archaea. The phylogeny of these groups revealed the presence of groups commonly found in soils and water samples, besides the occurrence of particular groups, possibly resulted from a speciation process in the mangrove environment. In general, results indicated that archaeal and bacterial communities are responsive to the mangroves contamination, and its alteration can also lead to differential nitrogen processing in these soils (AU)

FAPESP's process: 08/54013-8 - Diversity of archaea and bacteria involved in the nitrogen cycling in mangrove sediments
Grantee:Armando Cavalcante Franco Dias
Support Opportunities: Scholarships in Brazil - Doctorate