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Estimates of biological nitrogen fixation by tropical legume trees (Fabaceae) using 15N labeled fertilizer

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Author(s):
Luciana Della Coletta
Total Authors: 1
Document type: Master's Dissertation
Press: Piracicaba.
Institution: Universidade de São Paulo (USP). Centro de Energia Nuclear na Agricultura (CENA/STB)
Defense date:
Examining board members:
Luiz Antonio Martinelli; José Leonardo de Moraes Gonçalves; Takashi Muraoka
Advisor: Luiz Antonio Martinelli
Abstract

Legumes (Fabaceae) are abundant in the tropics and considered as of great interest as restorer of degraded areas, since most of them are woody and perennials, adapted to several Brazilian ecosystems and able to make associations with bacteria belonging to Rhizobium genus, which fix atmospheric nitrogen, and giving it to the plants in a reactive form. In this context, this study evaluated in a greenhouse experiment how nitrogen biological fixation (FBN) varied in three species of Fabaceae family according to the N-mineral addition in different doses and according to treatments including inoculation with bacteria of Rhizobium genus and arbuscular mycorrhizal fungi (AMF). The results obtained from the acetylene reduction activity indicate that the nodules were active in the last month of experiment in the three N2-fixing species. There was a wide variation in the plant N percentage derived from fixation (NPPfix). When plants grew in a soil where N availability was low, without application of this nutrient in the soil, the BNF was favored, ranging the NPPfix from 34 to 84%. On the contrary, it was possible to observe that FBN was inhibited in the pots where N additions were higher. Due to the use of unsterilized soil, the applied inoculants (bacteria and fungi) appear to have competed with the native soil microbiota, thus differences between inoculated and control (without inoculation) could not be established in this study (AU)