Molecular characterization of dengue type 3 isolated in Brazilian and Paraguay

Author(s)

Helda Liz Alfonso Castro

• Document type: Master's Dissertation
• Press: Ribeirão Preto.
• Institution: Universidade de São Paulo (USP). Faculdade de Ciências Farmacêuticas de Ribeirão Preto (PCARP/BC)
• Defense date: 2010-10-15

Examining board members

Victor Hugo Aquino Quintana; Simone Kashima Haddad; Mauricio Lacerda Nogueira

• Advisor: Victor Hugo Aquino Quintana

Abstract

ALFONSO CASTRO, H. L. Molecular characterization of dengue type 3 isolated in Brazilian and Paraguay. 2010. 105f. Dissertation (Master). Faculdade de Ciências Farmacêuticas de Ribeirão Preto Universidade de São Paulo, Ribeirão Preto, 2010. Infections of humans with dengue viruses (DENV), which belong to the genus Flavivirus(family, Flaviviridae), can be subclinical or cause illnesses ranging from a mild, flu-like syndrome with rash (dengue fever [DF]) to a severe and some times fatal disease, characterized by capillary leakage, thrombocytopenia, and sometimes hypovolemic shock (hemorrhagic dengue fever [DHF/DSS]). DENV are classified in four immunological distinct serotypes: DENV-1 to 4. Recently, a dramatically increase of DHF/DSS cases in the Americas have bee see, and this increase coincided with the introduction of the dengue virus type 3, genotype III. This virus causes a great epidemic in 2002 in the city of Rio de Janeiro and later, the virus spread in Paraguay. Phylogenetics and evolutionary studies have bee carried out with DENV-3 isolated worldwide, but using sequences partial genomic. In this work, we have analyzed the genetic diversity of DENV-3 of Brazilian and Paraguayan isolated, analyzing the complete sequences genomic. The Brazilian (n=9) and Paraguayan (n=3) isolated, were compared with 527 sequences deposited in the GeneBank. Theses isolated, belong to the American group of the genotype III. The phylogenetic analysis of complete genome of the DENV-3, confirmed the existence of three known genotypes and suggested the presence of other groups within each genotype named of the lineages, sub-lineages and clades. The genetic distance among the genotypes were of 7,3 to 7,5%, among the lineages of 3,2 to 5,3%, among the sub-lineages of 2,5 to 3,2% and among clades of 1,0 to 1,9%. The evolutionary rates of the viruses varied among 1,2x10-4 to 8,2x10-4 s/s/y. The age of the ancestral common more recent of the genotype I, possibly are among 1849-1945, the ancestral common of the genotype II, among 1916-1960 and the ancestral common more recent of the genotype III, among 1876-1923. The different genetic groups present motif of amino acids. These data could provide information for a better understanding of the evolution of theses viruses, and even for selection of candidate vaccine (AU)

• FAPESP's process: 07/06887-6 - Molecular Characterization of Dengue Type 3 isolated in Brazil and Paraguay
• Grantee: Helda Liz Alfonso Castro
• Support Opportunities: Scholarships in Brazil - Master