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| Author(s): |
Valter de Medeiros Winkel
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) |
| Defense date: | 2012-07-27 |
| Examining board members: |
Silvia Regina Ricci Lucas;
Archivaldo Reche Junior;
Lucas Campos de Sá Rodrigues
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| Advisor: | Silvia Regina Ricci Lucas |
| Abstract | |
Lymphoma belongs to a group of malignancies that have in common their origin in lymphoreticular cells, and is generally manifested in lymphoid tissues. In its evolution, there is a generalized reaction of the organism against a non-specific systemic conditions that compromises the homeostasis, known as acute phase response, which leads to a significant change in protein synthesis by the liver, resulting in an increase of some proteins known as acute phase proteins, and the most relevant are serum amyloid A, α-1 acid glycoprotein and C-reactive protein. This study was designed to define the electrophoretic profile of feline lymphoma and to evaluate serum concentrations of serum amyloid A (ASA), α-1 acid glycoprotein (GPA) and C-reactive protein (PCR) of these animals during chemotherapy, evaluated as possible indicators of disease remission. The study groups consisted of 20 clinically normal cats (control) and 16 cats with lymphoma (experimental). We excluded patients who had previous treatments and/or concomitant diseases. Electrophoresis of serum proteins was conducted on strips of cellulose acetate. For measurements of ASA, GPA and PCR we used commercial kits, which are then determined, in the control group, only once and, in the experimental group, at diagnosis and every 2 weeks during 3 months of treatment. Statistical analysis was performed with parametric tests, where unparied t test was used for comparisons between control and experimental groups at diagnosis and simple analysis of variance (ANOVA) test followed by Tukey\'s multiple comparisons to compare the experimental group in the diagnosis and weeks of treatment. There were significant differences between control and experimental groups at diagnosis of α-1 acid glycoprotein (p<0,0001), serum amyloid A (p=0,0028), C-reactive protein (p=0,0003), total globulin (p=0,0087), albumin: globulin (p<0,0001) and α-2 globulins (p=0,0082). When comparing the experimental group in the diagnosis and weeks of treatment was significant in the results of α-1 acid glycoprotein (p=0,0021) and serum amyloid A (p=0,0053), whereas C-reactive protein did not change significantly (p=0,4510). It is concluded that cats with lymphoma have an expressive acute phase response, characterized by increased in serum concentrations of serum amyloid A, α-1 acid glycoprotein and C-reactive protein, and the serum amyloid A and alpha-1 acid glycoprotein reference potential indicators of remission in those patients who were with their high concentrations at diagnosis. (AU) | |
| FAPESP's process: | 10/03815-7 - Evaluation of serum amyloid a, alfa-1 acid glycoprotein and C-reactive protein in feline lymphoma during chemotherapy |
| Grantee: | Valter de Medeiros Winkel |
| Support Opportunities: | Scholarships in Brazil - Master |