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| Author(s): |
Nathália Fernandes Gonçalves Machado
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2013-05-29 |
| Examining board members: |
José Gregorio Cabrera Gomez;
Regina Lúcia Baldini;
Gabriel Padilla Maldonado
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| Advisor: | José Gregorio Cabrera Gomez |
| Abstract | |
Polyhydroxyalkanoates (PHA) produced by Pseudomonas sp. LFM046 from carbohydrates and propionic acid contains monomers with odd and even number of carbons, representing a good model to study the propionate metabolism. Genes related to 2-methycytrate cycle were found in all Pseudomonas species organized in an operon prpRCAAcnMprpFD. Genes related to the methylmalonyl-CoA pathway were not found in Pseudomonas. Genes related to the propionic <font face=\"Symbol\">a-oxidation were found only in P. aeruginosa. Analysis of a prp mutant obtained by transposon insertion revealead that it was mutated in prpB gene, related to the 2-methylcytrate cycle. The analysis of mutants obtained using ultraviolet radiation showed that two should be affected in less relevant propionate metabolism pathways and other two in the activation of this organic acid. Experiments with labeled propionate (113C) demonstrated that the oxobutyrate, methylmalonil-CoA and 2-hydroxyglutarate pathways should not contribute to the propionate metabolism in Pseudomonas sp. LFM046. (AU) | |
| FAPESP's process: | 10/03162-3 - Study of propionate metabolism in Pseudomonas sp. LFM046 for the control in the composition of polyhydroxyalkanoates produced |
| Grantee: | Nathália Fernandes Gonçalves Machado |
| Support Opportunities: | Scholarships in Brazil - Master |