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| Author(s): |
Beatriz Villas Boas
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2013-05-09 |
| Examining board members: |
Jose Maria Alvarez Mosig;
Alexandre Salgado Basso;
Niels Olsen Saraiva Câmara
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| Advisor: | Jose Maria Alvarez Mosig |
| Abstract | |
Using FoxP3+GFP+ mice, we studied the phenotypic changes in spleen TREG along the early infection with Sylvio X10/4 T. cruzi parasites and evaluated their suppressive activity. Regarding expression (MFI) of different markers by TREG, we observed an increase in FoxP3, a progressive increase in CD25 expression, a small CTLA-4HIGH population, and a late increase in GITR expression. Besides, we observed increases in ICOS in the last days analyzed and increased expression of Fas and FasL. In addition, CD69 suffered a slight persistent augment. According to their suppressive activity upon proliferation of CD4+FoxP3- cells and upon IFN-<font face=\"Symbol\">g production, there were no major differences between TREGs cells from control and 7days infected mice. Moreover, responding 7d-CD4+FoxP3- showed similar susceptibility to suppression by control and 7days infected TREG. We demonstrate that during the early infection by T. cruzi TREG maintain their suppressive activity with increase in expression of some markers and responding CD4+ cells do not become resistant to suppression. (AU) | |
| FAPESP's process: | 10/04564-8 - Phenotypic and functional analysis of CD4+FoxP3+ T cells (regulatory Tcells) at the acute phase of murine infection by Trypanosoma cruzi |
| Grantee: | Beatriz Villas Bôas |
| Support Opportunities: | Scholarships in Brazil - Master |