Intracellular signaling triggered by streptozotocin subtoxic concentrations in neuro-2A cells: in vitro Alzheimers disease associated neurodegeneration model.

Author(s)

Caio Henrique Yokoyama Mazucanti

• Document type: Master's Dissertation
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2013-04-09

Examining board members

Cristoforo Scavone; Eliana Hiromi Akamine; Tania Marcourakis

• Advisor: Cristoforo Scavone

Abstract

Streptozotocin (STZ) has been used as an animal model for Diabetes and, more recently, its intracerebroventricular (icv) injection as an AD animal model. Our objective involves the assessment of the effects caused by subtoxic doses of STZ in a neuroblastoma cell line upon insulin signaling associated intracellular cascade. Results confirm STZ spontaneous NO donation and suggest that the drug is capable of modulating the intracellular cascade associated to insulin receptor due to the spontaneous donation of the free radical. Therefore, EROs production may play an important role in the mechanism linked to STZ induced neurotoxicity. Ultimately, it has been shown that STZ treatment, as well as insulin resistance alone, is capable of impair neurite outgrowth by NGF treatment. This work contributes for the elucidation of the mechanisms by which STZ icv injection may cause features similar to DA. (AU)

• FAPESP's process: 10/14090-3 - Effects of streptozotocin-induced neurodegeneration in neuro2A cells: in vitro model of neurodegeneration associated to Alzheimer's disease
• Grantee: Caio Henrique Yokoyama Mazucanti
• Support Opportunities: Scholarships in Brazil - Master