bcr-abl regulation of TRAIL, OSM, FAIM and NIPA expression.

Author(s)

Janine Marie Gisele Leroy

• Document type: Master's Dissertation
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2008-07-03

Examining board members

Joao Gustavo Pessini Amarante Mendes; Maria de Lourdes Lopes Ferrari Chauffaille; Sara Teresinha Ollala Saad

• Advisor: Joao Gustavo Pessini Amarante Mendes
• Field of knowledge: Biological Sciences - Immunology
• Indexed in: Banco de Dados Bibliográficos da USP-DEDALUS; Biblioteca Digital de Teses e Dissertações - USP
• Location: Universidade de São Paulo. Biblioteca do Instituto de Ciências Biomédicas; T-ICB BMI QW504; L619ce

Abstract

Chronic myelogenous leukemia (CML) is a stem cell disease characterized by the presence of the Bcr-Abl oncoprotein, which is the cause of the malignant transformation and the extreme resistance to apoptosis displayed by CML patients. Our aim was to analyze the alteration in global gene expression in Bcr-Abl expressing cells. Data obtained from microarray analysis showed significant up-regulation of nipa and faim in HL60.Bcr-Abl and down-regulation of osm and trail. These results were further confirmed by Real-Time PCR to nipa, faim and trail, but not for osm expression in HL-60.Bcr-Abl cells. To evaluate the potential of some of the modified genes as therapeutic targets or prognostic markers for CML, we also analyzed the expression of these genes in samples from CML patients. (AU)