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| Author(s): |
Rodrigo Mendes
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | Piracicaba. , ilustrações. |
| Institution: | Universidade de São Paulo (USP). Escola Superior de Agricultura Luiz de Queiroz (ESALA/BC) |
| Defense date: | 2008-03-03 |
| Examining board members: |
Aline Aparecida Pizzirani kleiner;
Joao Lucio de Azevedo;
Luis Eduardo Aranha Camargo;
Marli de Fatima Fiore;
Julia Kuklinsky Sobral
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| Advisor: | Aline Aparecida Pizzirani kleiner |
| Field of knowledge: | Biological Sciences - Genetics |
| Indexed in: | Banco de Dados Bibliográficos da USP-DEDALUS; Biblioteca Digital de Teses e Dissertações - USP |
| Location: | Universidade de São Paulo. Biblioteca Central da Escola Superior de Agricultura Luiz de Queiroz; t633.61; M538d |
| Abstract | |
In Brazil, the sugarcane is one of the most important cultivated crops. The sugarcane has received increased interest in the last years because of increase of the cultivated area and ethanol production to be used as biofuel. Considering its economical importance and the possibility of the use of the genetically modified plants; this crop has become the aim of current research for productivity and sustainability. In this context, the work on microbial communities associated with sugarcane is remarkable, because both, these communities play important functional role in the interaction with the plant, and studies performed in tropical conditions are limited as well. Fungal and bacterial communities associated with genetically modified sugarcane IMI-1 and its conventional isoline SP80-1842 were systematically isolated from plants cultivated in an experimental area in Piracicaba, SP, Brazil. The fungal communities associated with sugarcane were accessed by means of cultivation approach and PCR-denaturing gradient gel electrophoresis; the results revealed that these communities are not affected by transgeny. The microbial communities\' diversity was characterized and identified by using molecular tools. The fungus Fusarium moniliforme showed high frequency in association with the plant and it was observed that the endopolygalacturonase gene, pgIII, plays important role in order to determine the sort of association, either endophytic or pathogenic, between F. moniliforme and the host. The Burkholderia cepacia complex is an integral part of the endophytic bacterial community of sugarcane in Brazil and isolates of this complex are able to control F. moniliforme growth. Phylogenetic analyses indicated that the endophytic Burkholderia are closely related to clinical isolates of the B. cepacia complex isolated from cystic fibrosis patients. (AU) | |
| FAPESP's process: | 03/01436-5 - Effect of genetically modified sugarcane on endophytic and pathogenic isolates of Fusarium |
| Grantee: | Rodrigo Mendes |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |