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Prospecting, cloning and expression of genes of Burkholderia sp. with potential to phytopathogens control

Grant number: 11/11271-0
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): September 01, 2011
Effective date (End): August 31, 2015
Field of knowledge:Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
Principal Investigator:Welington Luiz de Araújo
Grantee:Emy Tiyo Mano
Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil


The genus Burkholderia is a Gram-negative bacteria, that present a high physiological and genetic diversity. Bacteria belonging to this genus are currently being subject to studies and have been widely used for many biotechnological applications, such as agricultural management (biocontrol agents, plant growth promoters), in bioremediation/biodegradation and as producer of important industrial, medicine and agriculture important molecules. In a previous study (Proc. FAPESP no.2008/52407-9), a mutant library was generated using the Burkholderia cenocepacea strain TC3.4.2R3. This bacterium was previously isolated endophytically from roots sugarcane (Proc. FAPESP 2002/14143-3), and used to control of plant pathogenic fungi and bacteria, as well as antibiotic producer. Some mutants, defective to pathogens control, were characterized showing that some genes close to putative pyocin, patatin, modification enzymes and transcriptional factors are related to antimicrobial activity and defense responses in plants. Thus, this project aims to contribute to a better understanding of the involvement of these B. cenocepacea genes in biological process related to pathogens control and synthesis of bioactivity molecules. For this, the complete Tn5 franking DNA will be sequenced, the putative ORFS will be identified, cloned and expressed. The proteins will be purified and partially characterized. These molecules and genes, if better characterized, could contribute to development of applicable products and using appropriate technologies the generation of agricultural formulations, recombinant micro-organisms, genetically modified plants, among other applications.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ROMERO-GUTIERREZ, KARENT J.; DOURADO, MANUELLA N.; GARRIDO, LEANDRO M.; OLCHANHESKI, LUIZ RICARDO; MANO, EMY T.; DINI-ANDREOTE, FRANCISCO; VALVANO, MIGUEL A.; ARAUJO, WELINGTON L.. Phenotypic traits of Burkholderia spp. associated with ecological adaptation and plant-host interaction. MICROBIOLOGICAL RESEARCH, v. 236, . (17/12510-4, 11/11271-0, 12/24217-6, 18/22127-6, 15/11563-1)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
MANO, Emy Tiyo. Prospection for genes of Burkholderia seminalis TC3.4.2R3 with potential for biocontrol.. 2015. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.

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