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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Phenotypic traits of Burkholderia spp. associated with ecological adaptation and plant-host interaction

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Author(s):
Romero-Gutierrez, Karent J. [1] ; Dourado, Manuella N. [1] ; Garrido, Leandro M. [1] ; Olchanheski, Luiz Ricardo [1] ; Mano, Emy T. [1] ; Dini-Andreote, Francisco [2, 3] ; Valvano, Miguel A. [4] ; Araujo, Welington L. [1]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Av Prof Lineu Presses, 1374 Biomed II, Cidade Univ, Sao Paulo 05508900, SP - Brazil
[2] Penn State Univ, Dept Plant Sci, University Pk, PA 16802 - USA
[3] Penn State Univ, Huck Inst Life Sci, University Pk, PA 16802 - USA
[4] Queens Univ Belfast, Wellcome Wolfson Inst Expt Med, Belfast BT9 7BL, Antrim - North Ireland
Total Affiliations: 4
Document type: Journal article
Source: MICROBIOLOGICAL RESEARCH; v. 236, JUN 2020.
Web of Science Citations: 0
Abstract

Burkholderia species have different lifestyles establishing mutualist or pathogenic associations with plants and animals. Changes in the ecological behavior of these bacteria may depend on genetic variations in response to niche adaptation. Here, we studied 15 Burkholderia strains isolated from different environments with respect to genetic and phenotypic traits. By Multilocus Sequence Analysis (MLSA) these isolates fell into 6 distinct groups. MLSA clusters did not correlate with strain antibiotic sensitivity, but with the bacterial ability to produce antimicrobial compounds and control orchid necrosis. Further, the B. seminalis strain TC3.4.2R3, a mutualistic bacterium, was inoculated into orchid plants and the interaction with the host was evaluated by analyzing the plant response and the bacterial oxidative stress response in planta. TC3.4.2R3 responded to plant colonization by increasing its own growth rate and by differential gene regulation upon oxidative stress caused by the plant, while reducing the plant's membrane lipid peroxidation. The bacterial responses to oxidative stress were recapitulated by bacterial exposure to the herbicide paraquat. We suggest that the ability of Burkholderia species to successfully establish in the rhizosphere correlates with genetic variation, whereas traits associated with antibiotic resistance are more likely to be categorized as strain specific. (AU)

FAPESP's process: 17/12510-4 - Endophytic microorganisms and the host plant: genes and metabolites involved in the interaction
Grantee:Welington Luiz de Araújo
Support Opportunities: Regular Research Grants
FAPESP's process: 11/11271-0 - Prospecting, cloning and expression of genes of Burkholderia sp. with potential to phytopathogens control
Grantee:Emy Tiyo Mano
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 12/24217-6 - Endophytic microorganisms: identification of genes related to microbial interactions
Grantee:Welington Luiz de Araújo
Support Opportunities: Regular Research Grants
FAPESP's process: 18/22127-6 - Burkholderia spp. and the environmental microbiome: are the interactions in lung and rhizo-sphere similar?
Grantee:Welington Luiz de Araújo
Support Opportunities: Regular Research Grants
FAPESP's process: 15/11563-1 - Molecular mechanisms related to the interaction between endophytic microorganisms and the host plant
Grantee:Welington Luiz de Araújo
Support Opportunities: Regular Research Grants