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Phenotypic and genome-wide association studies of the interaction between Burkholderia ambifaria RZ2MS16, a plant growth promoter rhizobacteria, and the tropical maize

Abstract

Burkholderia rhizobacteria may contribute to growth promotion of different crops. This is due to the ability that some bacterial strains have to produce phytohormones, siderophores and nitrogen fixation among other properties. Such processes increase the ability of some species of Burkholderia to be used as bio-fertlizers as a sustainable alternative to the use of chemical fertilizers. Burkholderia ambifaria, strain RZ2MS16, is a Plant Growth-Promoting Rhizobacteria (PGPR) isolated from guarana and it is able to increase the biomass of important crops including maize, soybean, sugarcane. However, little is known about the molecular mechanisms related to growth promotion ability of this strain as well its molecular interaction with the hosts. In other hand Also, preliminary studies have demonstrated that the symbiosis between nitrogen-fixing bacteria (NFB) and maize may be optimized by a co-inoculation using PGPR. A molecular study of the interaction PGPR-hosts and NFB as Azospirillum brasilense received till now little. Similarly, little is known about the host genetic herdability to traits related to the bacterial symbiosis the response to the nutrients that these microorganisms may offer. So, there is a great need to integrate genetic breeding programs with molecular studies of plant-microbe-interactions. Aiming an holistic view of the system PGPR-host-NFB the objectives of the present research are: i) understanding on the genomic level the mechanisms that allow B. ambifarma RZ2MS16 to act promoting increase maize growth; for this, it will be studied the bacterium genome using the knockout of genes related with plant growth promotion and the host-interaction. By transcriptomic, it will be investigated the interaction among the strain B. ambifaria, A. brasilense and tropical maize hybrids and ii) identification of the genetic control and associated SNPs (single nucleotide polymorphisms) related with the maize interaction and the co-inoculated bacteria. For this, it will be analyzed a complete diallel mating design among maize lines contrasting for nitrogen use efficiency, under low and high nitrogen availability in the soil and with and without bacterial inoculation. The phenotypic data will be evaluated using mixed models equations. For the identification of SNPs in linkage disequilibrium with target traits, molecular markers and phenotypic data will be evaluated by genome-wide association studies (GWAS). (AU)

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Scientific publications (8)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FERRAREZI, JESSICA APARECIDA; CARVALHO-ESTRADA, PAULA DE ALMEIDA; BATISTA, BRUNA DURANTE; ANICETO, RAFAEL MARTINS; PROHMANN TSCHOEKE, BRUNO AUGUSTO; DE MAIA ANDRADE, PEDRO AVELINO; LOPES, BRUNA DE MOURA; BONATELLI, MARIA LETICIA; ODISI, ESTACIO JUSSIE; AZEVEDO, JOAO LUCIO; QUECINE, MARIA CAROLINA. Effects of inoculation with plant growth-promoting rhizobacteria from the Brazilian Amazon on the bacterial community associated with maize in field. APPLIED SOIL ECOLOGY, v. 170, FEB 2022. Web of Science Citations: 0.
BATISTA, BRUNA DURANTE; DOURADO, MANUELLA NOBREGA; FIGUEREDO, EVERTHON FERNANDES; HORTENCIO, RENATA OCKNER; MARQUES, JOAO PAULO RODRIGUES; PIOTTO, FERNANDO ANGELO; BONATELLI, MARIA LETICIA; SETTLES, MATTHEW L.; AZEVEDO, JOAO LUCIO; QUECINE, MARIA CAROLINA. The auxin-producing Bacillus thuringiensis RZ2MS9 promotes the growth and modifies the root architecture of tomato (Solanum lycopersicum cv. Micro-Tom). Archives of Microbiology, v. 203, n. 7, p. 3869-3882, SEP 2021. Web of Science Citations: 2.
DE ALMEIDA, JAQUELINE RAQUEL; BONATELLI, MARIA LETICIA; BATISTA, BRUNA DURANTE; TEIXEIRA-SILVA, NATALIA SOUSA; MONDIN, MATEUS; DOS SANTOS, RAFAELA CRISTINA; BENTO, JOSE MAURICIO SIMOES; DE ALMEIDA HAYASHIBARA, CAROLINA ALESSANDRA; AZEVEDO, JOAO LUCIO; QUECINE, MARIA CAROLINA. Bacillus thuringiensis RZ2MS9, a tropical plant growth-promoting rhizobacterium, colonizes maize endophytically and alters the plant's production of volatile organic compounds during co-inoculation with Azospirillum brasilense Ab-V5. ENVIRONMENTAL MICROBIOLOGY REPORTS, AUG 2021. Web of Science Citations: 0.
BONATELLI, MARIA LETICIA; LI, JIE; DUMARESQ, ALINE; QUECINE, MARIA CAROLINA; SETTLES, MATTHEW L. Complete Genome Sequence of Bacillus sp. Strain RZ2MS9, a Multitrait Plant Growth Promoter. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 9, n. 31 JUL 2020. Web of Science Citations: 0.
VIDOTTI, MIRIAM SUZANE; LYRA, DANILO HOTTIS; MOROSINI, JULIA SILVA; GRANATO, ITALO STEFANINE CORREIA; QUECINE, MARIA CAROLINA; DE AZEVEDO, JOAO LUCIO; FRITSCHE-NETO, ROBERTO. Additive and heterozygous (dis)advantage GWAS models reveal candidate genes involved in the genotypic variation of maize hybrids to Azospirillum brasilense. PLoS One, v. 14, n. 9 SEP 19 2019. Web of Science Citations: 0.
VIDOTTI, MIRIAM SUZANE; MATIAS, FILIPE INACIO; ALVES, FILIPE COUTO; PEREZ-RODRIGUEZ, PAULINO; ALVARADO BELTRAN, GREGORIO; BURGUENO, JUAN; CROSSA, JOSE; FRITSCHE-NETO, ROBERTO. Maize responsiveness to Azospirillum brasilense: Insights into genetic control, heterosis and genomic prediction. PLoS One, v. 14, n. 6 JUN 7 2019. Web of Science Citations: 0.
BATISTA, BRUNA DURANTE; LACAVA, PAULO TEIXEIRA; FERRARI, ANDREA; TEIXEIRA-SILVA, NATALIA SOUSA; BONATELLI, MARIA LETICIA; TSUI, SARINA; MONDIN, MATEUS; KITAJIMA, ELLIOT WATANABE; PEREIRA, JOSE ODAIR; AZEVEDO, JOAO LUDO; QUECINE, MARIA CAROLINA. Screening of tropically derived, multi-trait plant growth- promoting rhizobacteria and evaluation of corn and soybean colonization ability. MICROBIOLOGICAL RESEARCH, v. 206, p. 33-42, 2018. Web of Science Citations: 7.
BATISTA, BRUNA DURANTE; TANIGUTI, LUCAS MITSUO; MONTEIRO-VITORELLO, CLAUDIA BARROS; AZEVEDO, JOAO LUCIO; QUECINE, MARIA CAROLINA. Draft Genome Sequence of Burkholderia ambifaria RZ2MS16, a Plant Growth-Promoting Rhizobacterium Isolated from Guarana, a Tropical Plant. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 4, n. 2 MAR-APR 2016. Web of Science Citations: 0.

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