Modulation of the interaction among growth promoting bacteria and corn by the quorum sensing system

• Grant number: 16/16868-8
• Support Opportunities: Scholarships in Brazil - Master
• Effective date (Start): November 01, 2016
• Effective date (End): May 31, 2018
• Field of knowledge: Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
• Principal Investigator: Maria Carolina Quecine Verdi
• Grantee: Jaqueline Raquel de Almeida
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Associated research grant: 14/16804-4 - Deciphering the molecular interaction between Puccinia psidii x Eucalyptus grandis through "omics" approaches, AP.JP
• Associated scholarship(s): 17/19361-4 - SCREENING AND IDENTIFICATION OF NEW GENES INVOLVED IN THE QUORUM SENSING SYSTEM, BE.EP.MS

Abstract

The corn is one of the most important in the world and also the Brazilian economy, which exported nearly 32 million tons in 2015. However, this culture requires an intense amount of chemical fertilizers, such as nitrogen. One of the alternatives to reduce the consumption of chemical nitrogen is the use of nitrogen fixing bacteria, such as Azospirillum brasilense case, already used commercially in corn. But we know that the microorganisms do not act alone, both on the ground and inside the plant there are millions of bacteria interacting in complex ways. In the search for other microorganisms capable of performing a similar task, the Bacillus sp. strain (RZ2MS9), isolated from the rhizosphere of guarana is reported as a promoter of plant growth in crops of corn and soybeans. But still there are few studies about the process of bacteria-plant interaction. Furthermore, a study with the RZ2MS9 strain, co-inoculated with Azospirillum brasilense (Ab-v5), showed positive results as to promote growth of corn, supporting the idea that co-inoculation with different microorganisms can further improve more plant development. This response to co-inoculation can be directly related to quorum sensing system (QS), the system communication between the bacteria, which is key to population control of bacterial species, in addition to control the formation of biofilms and regular various behaviors. Faced with this panorama, this project aims to monitor the lineage of the colonization process RZ2MS9 tagged with GFP in corn, in order to better understand the bacteria-plant interaction. Furthermore, from the silencing aiiA gene related to the QS system RZ2MS9 strain, is a study on influence during the process of colonization and plant growth promotion in maize, compared to co-inoculation of RZ2MS9 with the strains Azospirillum brasilense (Ab-v5) and Burkholderia ambifaria (RZ2MS16) also reported to promote plant growth of corn. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BATISTA, BRUNA DURANTE; TANIGUTI, LUCAS MITSUO; ALMEIDA, JAQUELINE RAQUEL; AZEVEDO, JOAO LUCIO; QUECINE, MARIA CAROLINA. Draft Genome Sequence of Multitrait Plant Growth-Promoting Bacillus sp. Strain RZ2MS9. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 4, n. 6, NOV-DEC 2016. (16/16868-8)

DE ALMEIDA, JAQUELINE RAQUEL; BONATELLI, MARIA LETICIA; BATISTA, BRUNA DURANTE; TEIXEIRA-SILVA, NATALIA SOUSA; MONDIN, MATEUS; DOS SANTOS, RAFAELA CRISTINA; BENTO, JOSE MAURICIO SIMOES; DE ALMEIDA HAYASHIBARA, CAROLINA ALESSANDRA; AZEVEDO, JOAO LUCIO; QUECINE, MARIA CAROLINA. Bacillus thuringiensis RZ2MS9, a tropical plant growth-promoting rhizobacterium, colonizes maize endophytically and alters the plant's production of volatile organic compounds during co-inoculation with Azospirillum brasilense Ab-V5. ENVIRONMENTAL MICROBIOLOGY REPORTS, v. 13, n. 6, AUG 2021. (16/16868-8, 15/01188-9, 14/50871-0)

BATISTA, BRUNA DURANTE; TANIGUTI, LUCAS MITSUO; ALMEIDA, JAQUELINE RAQUEL; AZEVEDO, JOAO LUCIO; QUECINE, MARIA CAROLINA. Draft Genome Sequence of Multitrait Plant Growth-Promoting Bacillus sp. Strain RZ2MS9. GENOME ANNOUNCEMENTS, v. 4, n. 6, p. 2-pg., 2016-11-01. (15/01188-9, 16/16868-8, 11/05204-8)

FIGUEREDO, EVERTHON FERNANDES; DA CRUZ, THIAGO ANGELO; ALMEIDA, JAQUELINE RAQUEL DE; BATISTA, BRUNA DURANTE; MARCON, JOELMA; ANDRADE, PEDRO AVELINO MAIA DE; HAYASHIBARA, CAROLINA ALESSANDRA DE ALMEIDA; ROSA, MAURICIO SANTOS; AZEVEDO, JOAO LUCIO; QUECINE, MARIA CAROLINA. The key role of indole-3-acetic acid biosynthesis by Bacillus thuringiensis RZ2MS9 in promoting maize growth revealed by the ipdC gene knockout mediated by the CRISPR-Cas9 system. MICROBIOLOGICAL RESEARCH, v. 266, p. 10-pg., 2022-10-12. (17/11026-1, 16/16868-8)

DE ALMEIDA, JAQUELINE RAQUEL; RIANO PACHON, DIEGO MAURICIO; FRANCESCHINI, LIVIA MARIA; DOS SANTOS, ISANELI BATISTA; FERRAREZI, JESSICA APARECIDA; MAIA DE ANDRADE, PEDRO AVELINO; MONTEIRO-VITORELLO, CLAUDIA BARROS; LABATE, CARLOS ALBERTO; QUECINE, MARIA CAROLINA. Revealing the high variability on nonconserved core and mobile elements of Austropuccinia psidii and other rust mitochondrial genomes. PLoS One, v. 16, n. 3, MAR 11 2021. (14/16804-4, 16/16868-8)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

ALMEIDA, Jaqueline Raquel de. Molecular mechanisms involved in the bacterial talking and maize growth promotion. 2018. Master's Dissertation - Universidade de São Paulo (USP). Escola Superior de Agricultura Luiz de Queiroz (ESALA/BC) Piracicaba.