Burkholderia spp. in sugarcane crop: the conundrum of antifungal production intrinsic antimicrobial resistance and pest control

• Grant number: 17/50447-2
• Support type: Regular Research Grants
• Duration: August 01, 2018 - August 31, 2020
• Field of knowledge: Agronomical Sciences - Agronomy - Plant Health
• Cooperation agreement: BBSRC, UKRI ; Newton Fund, with FAPESP as a partner institution in Brazil
• Principal researcher: Welington Luiz de Araújo
• Grantee: Welington Luiz de Araújo
• Principal researcher abroad: Miguel Valvano
• Institution abroad: Queen's University Belfast, Northern Ireland
• Home Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Burkholderia spp. is the main component of the sugarcane cultivable bacterial community. Members of this genus produce antifungal compounds and serve to phytopathogens control and plant growth promotion. However, Burkholderia cepacia complex (Bcc) are described as potential opportunistic agents of pulmonary infection and present intrinsic antimicrobial (iAMR) against different classes of antibiotics including antimicrobial peptides. iAMR is multifactorial and we have shown that production of hopanoids, polyamines and modifications of cell surface polysaccharides provide high-Level resistance, and increased bacterial virulence in the Galleria mellonella (Lepdoptera: Pyralidae) infection model. However, the relationship between iAMR and antifungal production is not established, but our previous results have shown that strains with high-Level antimicrobial resistance display higher capacity to inhibit pathogenic fungi. Thus, this proposal will explore a hypothesis that antifungal production triggers iAMR by influencing the production of membrane hopanoids and polyamines, and modification of cell surface polysaccharides. Our first aim will evaluate in Burkholderia spp. the relationship between the organization and expression of hopanoid genes cluster, iAMR and antifungal production. Also, we will evaluate the organization and expression of capsule and lipopolysaccharide gene clusters in Burkholderia spp. in relation to iAMR and the antifungal synthesis, as well as the expression of polyamine genes. Since we have observed that B. seminalis displays high virulence in the G. mellonella, our second aim will evaluate B. seminalis as a pest biocontrol agent against Diatraea saccharalis (Lepdoptera: Crambidae), a major pest for sugarcane. This proposal will address underpinning mechanisms associated to antifungal production and iAMR, and will enable us to translate this information into a practical biocontrol approaches. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ROMANO DE OLIVEIRA GONCALVES, PRISCILA JANE; HUME, CARMEN C. DENMAN; FERREIRA, ALMIR JOSE; TSUI, SARINA; BROCCHI, MARCELO; WREN, BRENDAN W.; ARAUJO, WELINGTON LUIZ. Environmental interactions are regulated by temperature in Burkholderia seminalis TC3.4.2R3. SCIENTIFIC REPORTS, v. 9, APR 2 2019. Web of Science Citations: 0.