Environmental interactions are regulated by temper... - BV FAPESP
Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Environmental interactions are regulated by temperature in Burkholderia seminalis TC3.4.2R3

Full text
Author(s):
Romano de Oliveira Goncalves, Priscila Jane [1] ; Hume, Carmen C. Denman [2] ; Ferreira, Almir Jose [1] ; Tsui, Sarina [1] ; Brocchi, Marcelo [3] ; Wren, Brendan W. [2] ; Araujo, Welington Luiz [1]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Dept Microbiol, BR-05508900 Sao Paulo - Brazil
[2] London Sch Hyg & Trop Med, Dept Pathogen Mol Biol, London WC1E 7HT - England
[3] Univ Estadual Campinas, Dept Genet Evolut Microbiol & Immunol, BR-13083862 Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 9, APR 2 2019.
Web of Science Citations: 0
Abstract

Burkholderia seminalis strain TC3.4.2R3 is an endophytic bacterium isolated from sugarcane roots that produces antimicrobial compounds, facilitating its ability to act as a biocontrol agent against phytopathogenic bacteria. In this study, we investigated the thermoregulation of B. seminalis TC3.4.2R3 at 28 degrees C (environmental stimulus) and 37 degrees C (host-associated stimulus) at the transcriptional and phenotypic levels. The production of biofilms and exopolysaccharides such as capsular polysaccharides and the biocontrol of phytopathogenic fungi were enhanced at 28 degrees C. At 37 degrees C, several metabolic pathways were activated, particularly those implicated in energy production, stress responses and the biosynthesis of transporters. Motility, growth and virulence in the Galleria mellonella larvae infection model were more significant at 37 degrees C. Our data suggest that the regulation of capsule expression could be important in virulence against G. mellonella larvae at 37 degrees C. In contrast, B. seminalis TC3.4.2R3 failed to cause death in infected BALB/c mice, even at an infective dose of 10(7) CFU.mL(-1). We conclude that temperature drives the regulation of gene expression in B. seminalis during its interactions with the environment. (AU)

FAPESP's process: 17/50447-2 - Burkholderia spp. in sugarcane crop: the conundrum of antifungal production intrinsic antimicrobial resistance and pest control
Grantee:Welington Luiz de Araújo
Support Opportunities: Regular Research Grants
FAPESP's process: 12/24217-6 - Endophytic microorganisms: identification of genes related to microbial interactions
Grantee:Welington Luiz de Araújo
Support Opportunities: Regular Research Grants
FAPESP's process: 13/03426-9 - Characterization of wcb cluster of B. seminalis strains TC3.4.2R3 and its role in the biosynthesis of antimicrobial compounds
Grantee:Priscila Jane Romano de Oliveira Gonçalves
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 14/24375-6 - Characterization of wcb cluster from b. seminalis TC3.4.2R3 and its role in the synthesis of antimicrobial compounds
Grantee:Priscila Jane Romano de Oliveira Gonçalves
Support Opportunities: Scholarships abroad - Research Internship - Doctorate
FAPESP's process: 17/12510-4 - Endophytic microorganisms and the host plant: genes and metabolites involved in the interaction
Grantee:Welington Luiz de Araújo
Support Opportunities: Regular Research Grants