Full text
| |
| Author(s): |
Marina Uchôa Wall Barbosa de Carvalho
Total Authors: 1
|
| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2013-06-19 |
| Examining board members: |
Antonio Condino Neto;
Cristina Maria Kokron;
Sonia Jancar Negro
|
| Advisor: | Antonio Condino Neto |
| Abstract | |
The APECED is a syndrome with autoantibodies and Candida albicans susceptibility. Our group has noted that AIRE protein is required for dectin-1 signaling, important against C. albicans. In this project, we investigate how the absence of AIRE influences in events for elimination of pathogen via dectin-1. We evaluated reactive oxygen species production, expression of NADPH oxidase molecules and phagocytosis by APECED pacient cells or AIRE silent THP-1 cells. We didnt observe differences in oxidative burst and expression of NADPH oxidase components by these cells and silent cells phagocytize less than wild-type cells. We observed no difference in flavocytochrome b558 and p40phox expression in paciente cells and control. In parallel, we showed that pacient cells has a decrease in burst oxidative and phagocytosis compared to control. Our results suggest that there is a defect in pathogen recognition via dectin-1, resulting in decrease on phagocytosis that can hamper their elimination. (AU) | |
| FAPESP's process: | 11/02751-8 - Phagocytosis and microbicidal activity against Candida albicans in THP-1 AIRE 1 deficient cells |
| Grantee: | Marina Uchôa Wall Barbosa de Carvalho |
| Support Opportunities: | Scholarships in Brazil - Master |