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| Author(s): |
Ciro Novaes Rosa Lino
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2013-08-23 |
| Examining board members: |
Anderson de Sá Nunes;
Niels Olsen Saraiva Câmara;
Jose Mauricio Sforcin
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| Advisor: | Anderson de Sá Nunes |
| Abstract | |
In order to successfully feed, hematophagous mosquitoes possess salivary components capable of regulating hemostasis and modulate the host immunity. However, the evaluation of the biological activities of the salivary molecules in the host still needs further investigation. In this study, we intend to characterize the biological activities of the AET-7393, a serpin that is present in the saliva of the females Aedes aegypti mosquitoes. Our data show that the recombinant AET-7393 serpin increases bleeding when inoculated in mice, but apparently this effect is not due to its interference on the coagulation cascade. In addition, AET-7393 is able to inhibit proteinase 3 and enhance the production of IL-1b. Finally, we observed the absence of modulatory effect on macrophage activation or inflammation, and that the presence of host anti-AET-7393 antibodies does not interfere in the life cycle of the mosquitoes. (AU) | |
| FAPESP's process: | 11/15569-3 - Characterization of the biological activity of Aedes aegypti salivary serpin AET-7393 |
| Grantee: | Ciro Novaes Rosa Lino |
| Support Opportunities: | Scholarships in Brazil - Master |