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| Author(s): |
Mariana Alba Zampol
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2013-09-19 |
| Examining board members: |
Mário Henrique de Barros;
Rodrigo da Silva Galhardo;
Regina Pekelmann Markus
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| Advisor: | Mário Henrique de Barros |
| Abstract | |
Parkinson\'s disease is a progressive neurodegenerative disorder often linked to mutation or expression alteration of the SNCA gene that encodes the a-synuclein (a-syn) protein. SNCA expression in Saccharomyces cerevisiae leads to deposition Lewy bodies that increase mitochondrial reactive oxygen species (ROS) production, one of the hallmarks of the disease. Hence, a possible treatment would be ROS neutralization through natural antioxidants, such as melatonin. Here we show that a-syn production is detrimental to yeast growth and respiration, similar to the phenotype observed during menadione oxidative challenge. Melatonin production or pharmacological supplementation confers antioxidant protection against oxidative agents, improving mitochondrial respiratory parameters from parkinsonian yeast grown with melatonin. Our studies point out to a promising role of melatonin in protecting yeast against a-syn and other species oxidative damage. (AU) | |
| FAPESP's process: | 11/03583-1 - Natural antioxidants action in Saccharomyces cerevisiae parkinsonian model |
| Grantee: | Mariana Alba Zampol |
| Support Opportunities: | Scholarships in Brazil - Master |