Genomic diversity analysis of geographic isolates of nucleopolyhedroviruses Anticarsia gemmatalis (AgMNPV).

Author(s)

Anderson Fernandes de Brito

• Document type: Master's Dissertation
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2013-09-24

Examining board members

Paolo Marinho de Andrade Zanotto; João Carlos Setubal; Robson Francisco de Souza

• Advisor: Paolo Marinho de Andrade Zanotto

Abstract

The Anticarsia gemmatalis multiple nucleopolyhedrovirus is employed as a biological control agent against velvetbean caterpillar (Anticarsia gemmatalis), and represents the most widely used viral bioinseticide in the world. Since baculovirus wild populations are genetically heterogeneous, this work evaluated the genomic diversity of 17 AgMNPV geographic isolates obtained from 1980 to 1990, in the Brazil, Argentina and Uruguay. The genotypes reconstructed evidenced the highly genomic colinearity conservation during AgMNPV evolutionary history, however, deletions and insertions of one or more bases were common. A new gene with possible lepidopteran origin was identified in two genotypes; and the gene bro-a is absence in many isolates. Genetic diversity analyses pointed that most AgMNPV genes shows little variation, and the highly diverse genes are in specific variable loci. The obtained results reinforce the perception that large DNA virus evolve mainly under low rates of substitution, and through gene duplications, gain and loss. (AU)

• FAPESP's process: 11/14537-0 - Genomic diversity analysis of geographic isolates of nucleopolyhedroviruses Anticarsia gemmatalis (AgMNPV)
• Grantee: Anderson Fernandes de Brito
• Support Opportunities: Scholarships in Brazil - Master