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| Author(s): |
Cristiano Jacob de Moraes
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2013-12-13 |
| Examining board members: |
Jose Alexandre Marzagao Barbuto;
Ana Paula Lepique;
Elaine Guadelupe Rodrigues
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| Advisor: | Jose Alexandre Marzagao Barbuto |
| Abstract | |
Dendritic cells ( DCs ) are critical in triggering antitumor immune response . But there are conditions in the tumor microenvironment that prevent this immunostimulatory function of DCs. This functional impairment appears to be the result of hyperactivation of STAT-3. The present study aimed to evaluate the ability of Ta1 to interfere in the activation of STAT-3. Then, monocytes and mo-DCs were treated or not with Ta1 and compared with the control, the STAT-3 inhibitor, JSI -124. We assessed the expression of surface molecules and the mo-DCs ability in stimulating allogeneic T lymphocytes. Ta1 did not affect the activation of STAT-3. In addition, Ta1 did not reproduce the effects found in mo-DCs from cancer patients. However, JSI -124 treatment led to changes in mo-DCs, that exhibited an inflammatory profile, with an increase of HLA-DR , CD86 and concomitant drop in PD- L1. Furthermore, we have shown that STAT-3 is involved in the expression of leukointergrins, since its inhibition resulted in down-regulation of expression level of this gene and protein molecules. (AU) | |
| FAPESP's process: | 11/15371-9 - STAT3 inhibition by thymosin alpha-1: Effective strategy for the functional recovery of cancer patients" monocyte-derived dendritic cells? |
| Grantee: | Cristiano Jacob de Moraes |
| Support Opportunities: | Scholarships in Brazil - Master |