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| Author(s): |
Rafael Barrera Salgueiro
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2013-10-08 |
| Examining board members: |
Maria Tereza Nunes;
José Donato Júnior;
Edilamar Menezes de Oliveira
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| Advisor: | Maria Tereza Nunes |
| Abstract | |
Exercise increases serum GH concentrations. During the same occurs elevation of lactate, which can stimulate hormone secretion. We intend to investigate whether lactate could be a factor that activates the somatotrophic axis. We used adult male Wistar rats (a) subjected to ip injection of 15 and 150 <font face=\"symbol\">mmols lactate during light or dark phase, and sacrificed after 30\', 60\' and 120\' injection (acute) or (b) subject to ev infusion of 15 and 150 <font face=\"symbol\">mmols lactate, in light or dark phase (chronic). Pituitary and their liver were collected to evaluate the GH expression (mRNA and protein) and IGF-I mRNA. The analysis was made by 2-way ANOVA. The injected lactate did not change serum lactate and glucose groups. No change in the parameters analyzed in the acute light group. The two lactate doses increased GH and IGF-I mRNA and GH serum content of the group acute dark. The lactate only increased GH mRNA and serum content of chronic dark group. In short, the lactate produced in the exercise may be one of the factors that activate the somatotropic axis. (AU) | |
| FAPESP's process: | 11/14961-7 - Effect of lactate infusion on the activity of GH-IGF-I axis in rats |
| Grantee: | Rafael Barrera Salgueiro |
| Support Opportunities: | Scholarships in Brazil - Master |