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| Author(s): |
Mônica Malheiros França
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2014-03-19 |
| Examining board members: |
Claudimara Ferini Pacicco Lotfi;
Jackson Cioni Bittencourt;
Erico Tosoni Costa;
Ana Amélia Fialho de Oliveira Hoff;
Ruy Gastaldoni Jaeger
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| Advisor: | Claudimara Ferini Pacicco Lotfi |
| Abstract | |
SF-1 and LRH-1 have played a critical role in steroid production, adrenal and gonads. Moreover, there are evidences that they have acted in tumorigenesis process in these organs. POD-1 is downregulated in adrenocortical carcinoma (ACC) it seems to regulate Sf-1. In this work, it has been to analyse the role of POD-1 in SF-1 and LRH-1 regulation in adrenocortical tumor cells. The POD-1 overexpression has reduced SF-1/SF-1 expression. However, there was an increase of LRH-1 gene expression due to SHP expression decrease which is negative regulate of LRH-1. The POD-1 and SF-1 gene expression in transfected cells with siRNA-POD-1 has shown POD-1 decrease and SF-1 increase emphasizing a regulatory mechanism between POD-1 and SF-1. By ChIP assay it was shown that POD-1 binded in SF-1 promoter E-box sequence. It was not characterized that POD-1 binded in LRH-1 promoter, although POD-1 can bind in SHP promoter E-box sequence. The reduction of SF-1 expression by POD-1 has decreased the StAR expression, however, it was not enough to change tumor cell proliferation. In summary, POD-1 must have a wider role as regulator of fator transcription which controls tumorigenese process being a possible candidate as tumor supressor gene in adrenocortical cells. (AU) | |
| FAPESP's process: | 10/00771-9 - The role and mechanism of the Sf-1 and Lrh-1 transcription factors regulation in the cell culture of humam adrenal tumor |
| Grantee: | Monica Malheiros França |
| Support Opportunities: | Scholarships in Brazil - Doctorate |