The baculovirus Anticarsia gemmatalis multiple nucleopolyhedrovirus proteome and the comparison of multiple isolated envelope proteins GP64.

Author(s)

Carla Torres Braconi

• Document type: Doctoral Thesis
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2013-11-01

Examining board members

Paolo Marinho de Andrade Zanotto; Ronaldo Zucatelli Mendonça; Enrique Mario Boccardo Pierulivo; Armando Morais Ventura; José Luiz Caldas Wolff

• Advisor: Paolo Marinho de Andrade Zanotto

Abstract

Baculoviridae are arthropod-specific viruses with more than 700 host insects, which produce two phenotypes: the budded virus (BV) and, the occlusion-derived virus (ODV) for intra and across host spread, respectively. Brazil uses the Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV) as a biological control agent of the velvet bean caterpillar (A. gemmatalis). The genome of the AgMNPV-2D carries 152 ORFs, 26 of which code for structural proteins. Herein, the structural proteins of AgMNPV-2D were analyzed by two mass spectrometry techniques. The additional objective was to compare the gene gp64. of different geographical populations by Sanger and next generation sequencing. This analysis allowed us to observe the substitutions of gp64 and refuted the notion of a geographical isolation of the samples. We also observed a total of 44 proteins of the ODV and 33 of the BV. Six new proteins were found in the ODV and seven in the BV. Furthermore, 11 cellular proteins were also identified, which are possibly assorted during viral morphogenesis. These findings may provide novel insights into AgMNPV biology and its host interaction, leading us to a better understanding about morphogenesis and also the associated factors of the viral multiplication. (AU)

• FAPESP's process: 09/16740-8 - Study of structural proteins of Anticarsia gemmatalis nucleopoliedrovirus (AgMNPV-2D) in different insect cell lines and comparison of the GP64 in geographic variants
• Grantee: Carla Torres Braconi
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)