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| Author(s): |
Luana Raposo de Melo Moraes Aps
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2013-11-12 |
| Examining board members: |
Luis Carlos de Souza Ferreira;
Enrique Mario Boccardo Pierulivo;
Rosa Maria Silva
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| Advisor: | Luis Carlos de Souza Ferreira |
| Abstract | |
Bacillus subtilis spores can be used as vaccine vehicles capable of to display or adsorb heterologous proteins in its surface. Recent studies have indicated that spores of B. subtilis confer a strong adjuvant effect for vaccines based on purified proteins administered by the parenteral route. In this scenario, the objective of this project is to evaluate the adjuvant role of B. subtilis spores e viabilize its use as DNA vaccine carriers of biolistic method (gene gun). In this methodology, the spores are used as gold microparticles substitutes usually employed, maintaining the ability to transfect eukaryotic cells in vitro and production of specific antibodies in C57BL / 6 mice immunized with the vaccine plasmid pgDE7h (encoding a non-toxic form of the E7oncoprotein of HPV-16). Additionally, spores of B. subtilis were able to activate dendritic cells in vitro when co-administered intramuscularly to pgDE7h, conferring an increase of 50% of anti-tumor protection in animals previously transplanted with the TC-1 tumor cell line (expressing the E7 protein of HPV-16) in a dose-dependent manner. (AU) | |
| FAPESP's process: | 11/13805-1 - Adjuvant effect of Bacillus subtilis spores co-administered with DNA vaccines |
| Grantee: | Luana Raposo de Melo Moraes Aps |
| Support Opportunities: | Scholarships in Brazil - Master |