Regulation of genes involved in oxidative stress response in Caulobacter crescentus.

Author(s)

Maristela Previato

• Document type: Master's Dissertation
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2013-11-26

Examining board members

Marilis do Valle Marques; Frederico José Gueiros Filho; Luis Eduardo Soares Netto

• Advisor: Marilis do Valle Marques

Abstract

Oxidative stress, caused by increased levels of reactive oxygen species, can lead to damage in all cellular components. Several enzymes, as subunit of alkyl hydroperoxide reductase (AhpC and AhpF) and superoxide dismutases (SOD), are responsible for removing ROS. Mechanisms of gene expression of C. crescentus for genes ahpC, sodA, sodB and sodC, were evaluated with transcription fusions with the lacZ reporter gene were constructed, allowing the quantification of expression by b-galactosidase activity assays, furthermore we analyze of the gene expression by qRT-PCR assay. The cultures were grown in PYE and M2 media, and gene expression was evaluated in the presence of hydrogen peroxide (H2O2), tert-butyl hydroperoxide (tBOOH), paraquat, menadione, pyrogallol, FeSO4 or DPPi. In C. crescentus ahpC is induced by peroxides and is under the control of OxyR. The SODs are mainly induced by superoxide, sodB and sodC are induction in stationary phase and are possibly under the control of sigma J, while the sodA gene is regulated by sigma F and sigma J. (AU)

• FAPESP's process: 11/04580-6 - Study of the regulation of genes involved in oxidative stress response in Caulobacter crescentus
• Grantee: Maristela Previato Mello
• Support Opportunities: Scholarships in Brazil - Master