Reproduction in Characidium schubarti (Teleostei: Characiformes): analysis of captive breeding and of genes related to sex differentiation (dmrt1, cyp19a1a and amh

Author(s)

Carolina Pereira Gomes

• Document type: Master's Dissertation
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Biociências (IBIOC/SB)
• Defense date: 2014-02-11

Examining board members

Lurdes Foresti de Almeida Toledo; Soraia Barreto Aguiar Fonteles; Yatiyo Yassuda

• Advisor: Lurdes Foresti de Almeida Toledo

Abstract

Teleost fishes represent about half of all vertebrate species already described. Such a rich diversity is reflected in different forms of sex determination, the main ones being triggered by environmental influences and genetic factors. Genes such as amh and dmrt1 are involved in this mechanism and have been characterized in several teleost fish species, they play an important role in the differentiation of teleost males whereas the cyp19a1a gene is related to the female sexual differentiation. Have been increasingly common in the literature, studies concerning the rule of genes in sex differentiation in teleosts, however pieces of information regarding the order Characiformes are scarce. Besides genetics, reproductive biology of teleost fishes also includes studies on the role of hormones and environment on reproduction, and also captive breeding. This thesis aims to analyze the aforementioned species in Characidium schubarti (Characiformes) genes for which little information exists on their reproductive biology literature featuring fragments of genes dmrt1 and cyp19a1a and the similarity of these with those already described in other orders of teleost fish . Moreover, it was possible to develop the project, obtaining relevant information regarding the role of the environment and exogenous hormones in reproduction of the species. It is expected that with the increase of research involving genes related to sex, in order to control the sexual gender for such an economically important fish can be successfully achieved from the control of the expression of factors of differentiation (AU)

• FAPESP's process: 11/15646-8 - Isolation and identification of molecular markers linked to the W chromosome and analysis of expression of genes related to gonadal sex differentiation in Characidium (Characiformes: Crenuchidae)
• Grantee: Carolina Pereira Gomes
• Support Opportunities: Scholarships in Brazil - Master