Full text
| |
| Author(s): |
Ana Paula Nascimento de Lima
Total Authors: 1
|
| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) |
| Defense date: | 2014-03-14 |
| Examining board members: |
Cristina de Oliveira Massoco Salles Gomes;
Frederico Azevedo da Costa Pinto;
Regina Helena da Silva
|
| Advisor: | Cristina de Oliveira Massoco Salles Gomes |
| Abstract | |
The effects of stress which are constant factors and important components in an individual\'s life cause changes on the immune system. Our hypothesis was acute restraint stress in three sessions on alternate days can change the expression of membrane markers and the function of dendritic cells (DCs) in BALB/c mice. Evaluate the effects of stress on the DC seemed to be relevant since these are important elements linking innate and adaptive immune responses, i,e., cells are highly specialized antigen-presenting with a unique capacity to activate T cells. First of all, behavioral assessment and dosages of hormones related to stress response were conducted to characterize and to validate the model of acute restraint stress. Then, splenic DCs and DCs generated from bone marrow were analyzed for expression of phenotypic markers CD11c, MHC-II, CD80, CD86, CD40 and CCR-7. The model of acute restraint stress changed the behavior and activate the HPA axis and the SNS. The analysis of phenotypic markers revealed an increased expression of CD40 on splenic DCs from stressed animals, however, there were no changes in BM-dDCs. The proliferation assay showed a change in the function of DCs in the coculture with splenocytes in a ratio of 1:1. Therefore, the present study indicated that acute restraint stress, conducted in three sessions on alternate days, is able to change some parameters related to the phenotype and function of splenic DCs. (AU) | |
| FAPESP's process: | 12/09332-3 - Effects of restraint stress on regulation of dendritic cells |
| Grantee: | Ana Paula Nascimento de Lima |
| Support Opportunities: | Scholarships in Brazil - Master |