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(EN) VECTOR FOR EXPRESSING FLUORESCENT PROTEINS IN HAPLOID CELLS, EXPRESSION CASSETTES AND METHOD FOR OBTAINING HAPLOIDS OF SACCHAROMYCES CEREVISIAE ON A LARGE SCALE
| Document type: | Patente |
| Inventor(s): | TEIXEIRA, Gleidson Silva; FURLAN, Monique; FARIAS, Alessandro dos Santos; PEREIRA, Gonçalo Amarante Guimarães |
| Applicant: | Universidade Estadual de Campinas (UNICAMP) |
| Deposit date: | October 2018, 04 |
| INPI register: | |
| IPC: | C12R 1/865 C12N 15/11 C12Q 1/04 C12N 15/64 C12N 15/81 C12N 1/19 |
Abstract
(EN) The present invention relates to a vector, called pMF_002 vector, and to the method of separating and collecting S. cerevisiae haploids using this vector in order to express fluorescent proteins (markers) in haploid (MATa and MATα) cells and thus distinguish them from each other and from tetrads and diploids, using a flow cytometer. For that purpose, this vector comprises the reporter genes EGFP (Enhanced Green Fluorescent Protein) and CyOFP1 (Cyan Orange Fluorescent Protein), the promoters STE2 (STErile2) and STE3 (STErile3), the terminators CYC1 (Cytochrome C1) and IDP1 (Isocitrate Dehydrogenase 1), and the backbone vector (pGS_004).
| FAPESP's process: | 16/12852-0 - Implementation of a tetrad dissection method for Saccharomyces cerevisiae based on flow cytometry |
| Grantee: | Monique Furlan |
| Principal Investigator: | Gonçalo Amarante Guimarães Pereira |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia (IB) |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |