Abstract
Several lines of evidence suggesting that conversion of pyruvate to acetyl-CoA limits ethanol titer. In many thermophilic bacteria, including C. thermocellum, this conversion is mediated by the pyruvate ferredoxin oxidoreductase (PFOR) enzyme. To identify metabolic bottlenecks in enzymes from C. thermocellum, we replaced the T. saccharolyticum enzymes with C. thermocellum enzymes. Of the…