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Richard John Ward

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Universidade de São Paulo (USP). Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP)  (Institutional affiliation for the last research proposal)
Birthplace: Inglaterra

Graduate of Physiology from the University of Sheffield, UK (1983) and PhD in Biophysics from The Oxford Research Unit Oxford (1987). Dr. Ward as a wide experience in Biophysics with emphasis on the structure/function relationships of proteins, with expertise in the following subjects: directed evolution of proteins, protein engineering of industrial enzymes, nanotechnology and biomimetic systems. (Source: Lattes Curriculum)

Research grants
Scholarships in Brazil
Scholarships abroad
FAPESP support in numbers * Updated November 21, 2020
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Use this Research Supported by FAPESP (BV/FAPESP) channel only to send messages referring to FAPESP-funded scientific projects.


 

 

 

 

Keywords used by the researcher
Scientific publications resulting from Research Grants and Scholarships under the grantee's responsibility (42)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

Publications33
Citations291
Cit./Article8.8
Data from Web of Science

VIEIRA, DAVI SERRADELLA; WARD, RICHARD JOHN. Conformation analysis of a surface loop that controls active site access in the GH11 xylanase A from Bacillus subtilis. Journal of Molecular Modeling, v. 18, n. 4, p. 1473-1479, . Web of Science Citations: 8.

FURTADO, G. P.; RIBEIRO, L. F.; LOURENZONI, M. R.; WARD, R. J.. A designed bifunctional laccase/beta-1,3-1,4-glucanase enzyme shows synergistic sugar release from milled sugarcane bagasse. PROTEIN ENGINEERING DESIGN & SELECTION, v. 26, n. 1, p. 15-23, . Web of Science Citations: 18. (10/18850-2, 07/01623-0)

DE CARVALHO, DANIELLA ROMANO; CARLI, SIBELI; MELEIRO, LUANA PARRAS; ROSA, JOSE CESAR; CAVALCANTE DE OLIVEIRA, ARTHUR HENRIQUE; JORGE, JOAO ATILIO; MELO FURRIEL, ROSA PRAZERES. A halotolerant bifunctional beta-xylosidase/alpha-L-arabinofuranosidase from Colletotrichum graminicola: Purification and biochemical characterization. International Journal of Biological Macromolecules, v. 114, p. 741-750, . Web of Science Citations: 7. (14/14415-0, 17/13734-3, 16/17582-0)

FURTADO, GILVAN P.; SANTOS, CAMILA R.; CORDEIRO, ROSA L.; RIBEIRO, LUCAS F.; DE MORAES, LUIZ A. B.; DAMASIO, ANDR R. L.; POLIZELI, MARIA DE LOURDES T. M.; LOURENZONI, MARCOS R.; MURAKAMI, MARIO T.; WARD, RICHARD J.. Enhanced xyloglucan-specific endo-beta-1,4-glucanase efficiency in an engineered CBM44-XegA chimera. Applied Microbiology and Biotechnology, v. 99, n. 12, p. 5095-5107, . Web of Science Citations: 9. (10/18850-2, 14/07135-1, 10/07133-8, 13/13309-0, 12/20549-4, 13/18910-3)

RIBEIRO, LILIANE F. C.; DE LUCAS, ROSYMAR C.; VITCOSQUE, GABRIELA L.; RIBEIRO, LUCAS F.; WARD, RICHARD J.; RUBIO, MARCELO V.; DAMASIO, ANDRE R. L.; SQUINA, FABIO M.; GREGORY, REBECCA C.; WALTON, PAUL H.; et al. A novel thermostable xylanase GH10 from Malbranchea pulchella expressed in Aspergillus nidulans with potential applications in biotechnology. BIOTECHNOLOGY FOR BIOFUELS, v. 7, . Web of Science Citations: 28. (10/18850-2, 12/02017-5, 10/07133-8, 12/12859-3, 10/10184-3, 13/18910-3, 08/57908-6)

FONSECA-MALDONADO, RAQUEL; VIEIRA, DAVI SERRADELLA; ALPONTI, JULIANA SANCHEZ; BONNEIL, ERIC; THIBAULT, PIERRE; WARD, RICHARD JOHN. Engineering the Pattern of Protein Glycosylation Modulates the Thermostability of a GH11 Xylanase. Journal of Biological Chemistry, v. 288, n. 35, p. 25522-25534, . Web of Science Citations: 31. (10/18850-2, 08/57908-6)

SILVA, SERGIO BERGAMACHI; PINHEIRO, MATHEUS PINTO; FUZO, CARLOS ALESSANDRO; SILVA, SAMUEL REGHIM; FERREIRA, TATIANE LOPES; LOURENZONI, MARCOS ROBERTO; NONATO, M. CRISTINA; VIEIRA, DAVI SERRADELLA; WARD, RICHARD JOHN. The role of local residue environmental changes in thermostable mutants of the GH11 xylanase from Bacillus subtilis. International Journal of Biological Macromolecules, v. 97, p. 574-584, . Web of Science Citations: 2. (12/01066-2, 10/18850-2)

RIBEIRO, LUCAS FERREIRA; NICHOLES, NATHAN; TULLMAN, JENNIFER; COSTA RIBEIRO, LILIANE FRAGA; FUZO, CARLOS ALESSANDRO; VIEIRA, DAVI SERRADELLA; FURTADO, GILVAN PESSOA; OSTERMEIER, MARC; WARD, RICHARD JOHN. Insertion of a xylanase in xylose binding protein results in a xylose-stimulated xylanase. BIOTECHNOLOGY FOR BIOFUELS, v. 8, . Web of Science Citations: 11. (10/18850-2)

APARECIDA ARAGÃO‚ E.; CHIOATO‚ L.; WARD‚ R.J.. Permeabilization of E. coli K12 inner and outer membranes by bothropstoxin-I‚ A LYS49 phospholipase A2 from Bothrops jararacussu. Toxicon, v. 51, n. 4, p. 538-546, .

ALVES, LUANA DE FATIMA; MELEIRO, LUANA PARRAS; SILVA, ROBERTO N.; WESTMANN, CAUA ANTUNES; GUAZZARONI, MARIA-EUGENIA. Novel Ethanol- and 5-Hydroxymethyl Furfural-Stimulated beta-Glucosidase Retrieved From a Brazilian Secondary Atlantic Forest Soil Metagenome. FRONTIERS IN MICROBIOLOGY, v. 9, . Web of Science Citations: 2. (15/04309-1, 16/05472-6, 16/17582-0, 16/06323-4)

CARNEIRO, LARA A. B. C.; WARD, RICHARD J.. Functionalization of paramagnetic nanoparticles for protein immobilization and purification. Analytical Biochemistry, v. 540, p. 45-51, . Web of Science Citations: 5. (16/15708-7, 10/18850-2, 12/24147-8)

MELEIRO, LUANA PARRAS; SANTOS SALGADO, JOSE CARLOS; MALDONADO, RAQUEL FONSECA; CARLI, SIBELI; BERALDO MORAES, LUIZ ALBERTO; WARD, RICHARD JOHN; JORGE, JOAO ATILIO; MELO FURRIEL, ROSA PRAZERES. Engineering the GH1 beta-glucosidase from Humicola insolens: Insights on the stimulation of activity by glucose and xylose. PLoS One, v. 12, n. 11, . Web of Science Citations: 9. (14/14415-0, 10/18850-2, 16/17582-0)

CHIOATO, LUCIMARA; ARAGAO, ELISANGELA APARECIDA; FERREIRA, TATIANA LOPES; WARD, RICHARD J.. Active site mutants of human secreted Group IIA Phospholipase A(2) lacking hydrolytic activity retain their bactericidal effect. Biochimie, v. 94, n. 1, p. 132-136, . Web of Science Citations: 4.

SANTOS SALGADO, JOSE CARLOS; MELEIRO, LUANA PARRAS; CARLI, SIBELI; WARD, RICHARD JOHN. Glucose tolerant and glucose stimulated beta-glucosidases - A review. Bioresource Technology, v. 267, p. 704-713, . Web of Science Citations: 15. (17/13734-3, 16/17582-0, 14/50884-5)

CARLI, SIBELI; MELEIRO, LUANA PARRAS; WARD, RICHARD JOHN. Biochemical and kinetic characterization of the recombinant GH28 Stereum purpureum endopolygalacturonase and its biotechnological application. International Journal of Biological Macromolecules, v. 137, p. 469-474, . Web of Science Citations: 0. (17/13734-3, 16/17582-0)

CHIOATO‚ L.; WARD‚ R.J.. Mapping structural determinants of biological activities in snake venom phospholipases A2 by sequence analysis and site directed mutagenesis. Toxicon, v. 42, n. 8, p. 869-883, .

WARD‚ R.J.; CHIOATO‚ L.; DE OLIVEIRA‚ A.H.C.; RULLER‚ R.; SÁ‚ J.M.. Active-site mutagenesis of a Lys49-phospholipase A2: biological and membrane-disrupting activities in the absence of catalysis.. Biochemical Journal, v. 362, n. Pt 1, p. 89, .

RULLER‚ R.; ARAGAO‚ EA; CHIOATO‚ L.; FERREIRA‚ T.L.; DE OLIVEIRA‚ AHC; SA‚ JM; WARD‚ RJ. A predominant role for hydrogen bonding in the stability of the homodimer of bothropstoxin-I‚ A lysine 49-phospholipase A2. Biochimie, v. 87, n. 11, p. 993-1003, .

CARNEIRO, LARA A. B. C.; YU, LI; DUPREE, PAUL; WARD, RICHARD J.. Characterization of a beta-galactosidase from Bacillus subtilis with transgalactosylation activity. International Journal of Biological Macromolecules, v. 120, n. A, p. 279-287, . Web of Science Citations: 2. (10/18850-2, 12/24147-8)

FONSECA-MALDONADO, RAQUEL; FERREIRA, TATIANA LOPES; WARD, RICHARD J.. The bactericidal effect of human secreted group IID phospholipase A(2) results from both hydrolytic and non-hydrolytic activities. Biochimie, v. 94, n. 6, p. 1437-1440, . Web of Science Citations: 3.

ARAGAO, E. A.; CHIOATO, L.; FERREIRA, T. L.; MEDEIROS, A. I. DE; SECATTO, A.; FACCIOLI, L. H.; WARD, R. J.. Suramin inhibits macrophage activation by human group IIA phospholipase A(2), but does not affect bactericidal activity of the enzyme. Inflammation Research, v. 58, n. 4, p. 210-217, . Web of Science Citations: 2.

FONSECA-MALDONADO, RAQUEL; FERREIRA, TATIANA LOPES; WARD, RICHARD J.. The bactericidal effect of human secreted group IID phospholipase A(2) results from both hydrolytic and non-hydrolytic activities. Biochimie, v. 94, n. 6, p. 1437-1440, . Web of Science Citations: 3.

CHIOATO‚ L.; DE OLIVEIRA‚ A.H.C.; RULLER‚ R.; SÁ‚ J.M.; WARD‚ R.J.. Distinct sites for myotoxic and membrane-damaging activities in the C-terminal region of a Lys49-phospholipase A2.. Biochemical Journal, v. 366, n. Pt 3, p. 971, .

SÁ‚ J.M.; CHIOATO‚ L.; FERREIRA‚ T.L.; DE OLIVEIRA‚ A.H.C.; RULLER‚ R.; ROSA‚ J.C.; GREENE‚ L.J.; WARD‚ R.J.. Topology of the substrate-binding site of a Lys49-phospholipase A2 influences Ca2+-independent membrane-damaging activity. Biochemical Journal, v. 382, n. Pt 1, p. 191, .

VIEIRA, DAVI SERRADELLA; WARD, RICHARD JOHN. Conformation analysis of a surface loop that controls active site access in the GH11 xylanase A from Bacillus subtilis. Journal of Molecular Modeling, v. 18, n. 4, p. 1473-1479, . Web of Science Citations: 8.

FURTADO, G. P.; RIBEIRO, L. F.; LOURENZONI, M. R.; WARD, R. J.. A designed bifunctional laccase/beta-1,3-1,4-glucanase enzyme shows synergistic sugar release from milled sugarcane bagasse. PROTEIN ENGINEERING DESIGN & SELECTION, v. 26, n. 1, p. 15-23, . Web of Science Citations: 18. (10/18850-2, 07/01623-0)

DE CARVALHO, DANIELLA ROMANO; CARLI, SIBELI; MELEIRO, LUANA PARRAS; ROSA, JOSE CESAR; CAVALCANTE DE OLIVEIRA, ARTHUR HENRIQUE; JORGE, JOAO ATILIO; MELO FURRIEL, ROSA PRAZERES. A halotolerant bifunctional beta-xylosidase/alpha-L-arabinofuranosidase from Colletotrichum graminicola: Purification and biochemical characterization. International Journal of Biological Macromolecules, v. 114, p. 741-750, . Web of Science Citations: 7. (14/14415-0, 17/13734-3, 16/17582-0)

FURTADO, GILVAN P.; SANTOS, CAMILA R.; CORDEIRO, ROSA L.; RIBEIRO, LUCAS F.; DE MORAES, LUIZ A. B.; DAMASIO, ANDR R. L.; POLIZELI, MARIA DE LOURDES T. M.; LOURENZONI, MARCOS R.; MURAKAMI, MARIO T.; WARD, RICHARD J.. Enhanced xyloglucan-specific endo-beta-1,4-glucanase efficiency in an engineered CBM44-XegA chimera. Applied Microbiology and Biotechnology, v. 99, n. 12, p. 5095-5107, . Web of Science Citations: 9. (10/18850-2, 14/07135-1, 10/07133-8, 13/13309-0, 12/20549-4, 13/18910-3)

RIBEIRO, LILIANE F. C.; DE LUCAS, ROSYMAR C.; VITCOSQUE, GABRIELA L.; RIBEIRO, LUCAS F.; WARD, RICHARD J.; RUBIO, MARCELO V.; DAMASIO, ANDRE R. L.; SQUINA, FABIO M.; GREGORY, REBECCA C.; WALTON, PAUL H.; et al. A novel thermostable xylanase GH10 from Malbranchea pulchella expressed in Aspergillus nidulans with potential applications in biotechnology. BIOTECHNOLOGY FOR BIOFUELS, v. 7, . Web of Science Citations: 28. (10/18850-2, 12/02017-5, 10/07133-8, 12/12859-3, 10/10184-3, 13/18910-3, 08/57908-6)

FONSECA-MALDONADO, RAQUEL; VIEIRA, DAVI SERRADELLA; ALPONTI, JULIANA SANCHEZ; BONNEIL, ERIC; THIBAULT, PIERRE; WARD, RICHARD JOHN. Engineering the Pattern of Protein Glycosylation Modulates the Thermostability of a GH11 Xylanase. Journal of Biological Chemistry, v. 288, n. 35, p. 25522-25534, . Web of Science Citations: 31. (10/18850-2, 08/57908-6)

SANTOS SALGADO, JOSE CARLOS; MELEIRO, LUANA PARRAS; CARLI, SIBELI; WARD, RICHARD JOHN. Glucose tolerant and glucose stimulated beta-glucosidases - A review. Bioresource Technology, v. 267, p. 704-713, . Web of Science Citations: 15. (17/13734-3, 16/17582-0, 14/50884-5)

CARLI, SIBELI; MELEIRO, LUANA PARRAS; WARD, RICHARD JOHN. Biochemical and kinetic characterization of the recombinant GH28 Stereum purpureum endopolygalacturonase and its biotechnological application. International Journal of Biological Macromolecules, v. 137, p. 469-474, . Web of Science Citations: 0. (17/13734-3, 16/17582-0)

ALVES, LUANA DE FATIMA; MELEIRO, LUANA PARRAS; SILVA, ROBERTO N.; WESTMANN, CAUA ANTUNES; GUAZZARONI, MARIA-EUGENIA. Novel Ethanol- and 5-Hydroxymethyl Furfural-Stimulated beta-Glucosidase Retrieved From a Brazilian Secondary Atlantic Forest Soil Metagenome. FRONTIERS IN MICROBIOLOGY, v. 9, . Web of Science Citations: 2. (15/04309-1, 16/05472-6, 16/17582-0, 16/06323-4)

CARNEIRO, LARA A. B. C.; WARD, RICHARD J.. Functionalization of paramagnetic nanoparticles for protein immobilization and purification. Analytical Biochemistry, v. 540, p. 45-51, . Web of Science Citations: 5. (16/15708-7, 10/18850-2, 12/24147-8)

MELEIRO, LUANA PARRAS; SANTOS SALGADO, JOSE CARLOS; MALDONADO, RAQUEL FONSECA; CARLI, SIBELI; BERALDO MORAES, LUIZ ALBERTO; WARD, RICHARD JOHN; JORGE, JOAO ATILIO; MELO FURRIEL, ROSA PRAZERES. Engineering the GH1 beta-glucosidase from Humicola insolens: Insights on the stimulation of activity by glucose and xylose. PLoS One, v. 12, n. 11, . Web of Science Citations: 9. (14/14415-0, 10/18850-2, 16/17582-0)

SILVA, SERGIO BERGAMACHI; PINHEIRO, MATHEUS PINTO; FUZO, CARLOS ALESSANDRO; SILVA, SAMUEL REGHIM; FERREIRA, TATIANE LOPES; LOURENZONI, MARCOS ROBERTO; NONATO, M. CRISTINA; VIEIRA, DAVI SERRADELLA; WARD, RICHARD JOHN. The role of local residue environmental changes in thermostable mutants of the GH11 xylanase from Bacillus subtilis. International Journal of Biological Macromolecules, v. 97, p. 574-584, . Web of Science Citations: 2. (12/01066-2, 10/18850-2)

RIBEIRO, LUCAS FERREIRA; NICHOLES, NATHAN; TULLMAN, JENNIFER; COSTA RIBEIRO, LILIANE FRAGA; FUZO, CARLOS ALESSANDRO; VIEIRA, DAVI SERRADELLA; FURTADO, GILVAN PESSOA; OSTERMEIER, MARC; WARD, RICHARD JOHN. Insertion of a xylanase in xylose binding protein results in a xylose-stimulated xylanase. BIOTECHNOLOGY FOR BIOFUELS, v. 8, . Web of Science Citations: 11. (10/18850-2)

FURTADO, GILVAN P.; SANTOS, CAMILA R.; CORDEIRO, ROSA L.; RIBEIRO, LUCAS F.; DE MORAES, LUIZ A. B.; DAMASIO, ANDR R. L.; POLIZELI, MARIA DE LOURDES T. M.; LOURENZONI, MARCOS R.; MURAKAMI, MARIO T.; WARD, RICHARD J.. Enhanced xyloglucan-specific endo-beta-1,4-glucanase efficiency in an engineered CBM44-XegA chimera. Applied Microbiology and Biotechnology, v. 99, n. 12, p. 5095-5107, . Web of Science Citations: 9. (10/18850-2, 14/07135-1, 10/07133-8, 13/13309-0, 12/20549-4, 13/18910-3)

RIBEIRO, LILIANE F. C.; DE LUCAS, ROSYMAR C.; VITCOSQUE, GABRIELA L.; RIBEIRO, LUCAS F.; WARD, RICHARD J.; RUBIO, MARCELO V.; DAMASIO, ANDRE R. L.; SQUINA, FABIO M.; GREGORY, REBECCA C.; WALTON, PAUL H.; et al. A novel thermostable xylanase GH10 from Malbranchea pulchella expressed in Aspergillus nidulans with potential applications in biotechnology. BIOTECHNOLOGY FOR BIOFUELS, v. 7, . Web of Science Citations: 28. (10/18850-2, 12/02017-5, 10/07133-8, 12/12859-3, 10/10184-3, 13/18910-3, 08/57908-6)

DE CARVALHO, DANIELLA ROMANO; CARLI, SIBELI; MELEIRO, LUANA PARRAS; ROSA, JOSE CESAR; CAVALCANTE DE OLIVEIRA, ARTHUR HENRIQUE; JORGE, JOAO ATILIO; MELO FURRIEL, ROSA PRAZERES. A halotolerant bifunctional beta-xylosidase/alpha-L-arabinofuranosidase from Colletotrichum graminicola: Purification and biochemical characterization. International Journal of Biological Macromolecules, v. 114, p. 741-750, . Web of Science Citations: 7. (14/14415-0, 17/13734-3, 16/17582-0)

FONSECA-MALDONADO, RAQUEL; VIEIRA, DAVI SERRADELLA; ALPONTI, JULIANA SANCHEZ; BONNEIL, ERIC; THIBAULT, PIERRE; WARD, RICHARD JOHN. Engineering the Pattern of Protein Glycosylation Modulates the Thermostability of a GH11 Xylanase. Journal of Biological Chemistry, v. 288, n. 35, p. 25522-25534, . Web of Science Citations: 31. (10/18850-2, 08/57908-6)

CHIOATO, LUCIMARA; ARAGAO, ELISANGELA APARECIDA; FERREIRA, TATIANA LOPES; WARD, RICHARD J.. Active site mutants of human secreted Group IIA Phospholipase A(2) lacking hydrolytic activity retain their bactericidal effect. Biochimie, v. 94, n. 1, p. 132-136, . Web of Science Citations: 4.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

ALVES, Luana de Fátima. Criação de uma enzima multifuncional feruloil esterase/acetil-xilano esterase por desenho racional. Dissertação (Mestrado) -  Faculdade de Medicina de Ribeirão Preto.  Universidade de São Paulo (USP).  Ribeirão Preto.  (14/07468-0

ARAGÃO, Elisângela Aparecida. Efeito da suramina na atividade da fosfolipase A2 secretada humana do grupo IIA. Tese (Doutorado) -  Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto.  Universidade de São Paulo (USP).  Ribeirão Preto.  (05/50379-0

MOLINA, Gustavo Avelar. Caracterização biofísica da dinâmica catalítica de uma xilanase GH11. Dissertação (Mestrado) -  Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto.  Universidade de São Paulo (USP).  Ribeirão Preto.  (14/06319-1

ALVES, Adriana Rodrigues. Investigação da relação entre estrutura e função em uma fospolipase PLA2 utilizando mutagênese sítio-dirigida.  101 f. Dissertação (Mestrado) -  Instituto de Biociências, Letras e Ciências Exatas.  Universidade Estadual Paulista.  São José do Rio Preto.  (94/01580-8

ARAGÃO, Elisângela Aparecida. Efeito bactericida de Fosfolipases A2-Lys49: o papel da região C-terminal na atividade de Bothropstoxina-I em membranas biológicas e artificiais.. Dissertação (Mestrado) -  Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto.  Universidade de São Paulo (USP).  Ribeirão Preto.  (02/12097-4

BUGS, Raquel Kely Bortoleto. Transformações estruturais e função de fosfolipase.  115 f. Tese (Doutorado) -  Universidade Estadual Paulista "Júlio de Mesquita Filho" Instituto de Biociências, Letras e Ciências Exatas.  São José do Rio Preto.  (97/02749-4

Patent applications

PARTÍCULAS FERROMAGNÉTICAS E SEUS USOS BR1020160210950 - Universidade de São Paulo (USP). Richard John Ward; Lara Aparecida Buffoni de Campos Carneiro - September 2016, 13

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