Luis Lamberti Pinto da Silva

• Abstract

  The Oropouche virus (OROV), a member of the Peribunyaviridae family, has become an alarming public health threat in Latin America, with a marked increase in infection cases between 2023 and 2024. Formerly restricted to the Amazon region, the virus has now spread across all regions of Brazil, including major urban centers previously unaffected. This recent epidemiological expansion has rai…

  Study to Advance the Understanding of the Replication Mechanisms of Oropouche Virus and Other Bunyaviruses in Human Cells., AP.R

• Abstract

  Electron microscopy (EM) is a crucial tool in basic and clinical studies in biomedicine, allowing the detailed visualization of cellular and molecular structures at nanometric scales. The evolution of the technique includes cryo-electron microscopy (Cryo-EM), which preserves samples in their native state, enabling 3D electron microscopy, which reconstructs three-dimensional structures. 3D…

  EMU: Acquisition of equipment to update and expand the research support services provided by the FMRP-USP Multi-User Electron Microscopy Laboratory., AP.INFRA7.PM

• Abstract

  The emergence of life diversity and complexity has been facilitated by intercellular communication, which is partially supported by a highly conserved pathway known as the conventional secretory pathway. This pathway involves the secretion of proteins containing a signal peptide through the endoplasmic reticulum-Golgi apparatus-final destination route. Although some questions remain open,…

  The exquisite biophysics of the unconventional protein secretion: old and new looks to old and new problems, AP.TEM

• Abstract

  Oropouche fever, a debilitating illness common in South America, is caused by Oropouchevirus (OROV), an arbovirus. OROV belongs to the Peribunyaviridae family, a large group ofRNA viruses. Little is known about the biology of Peribunyaviridae in host cells, especiallyassembly and egress processes. Our research reveals that the small GTPase Rab27amediates intracellular transport of OROV in…

  Rab27a GTPase e seu efetor Myosin Va são fatores hospedeiros necessários para saída celular eficiente do virus Oropouche., PUB.ART

• Abstract

  The Zika virus (ZIKV) and the Oropouche virus (OROV) are emerging arboviruses of great importance to public health, and due to global climate changes, they have the potential to spread to new tropical and subtropical regions. While ZIKV caused a severe epidemic in Brazil, OROV causes recurrent and recent outbreaks in the Caribbean and tropical regions of Latin America. Both viruses, despi…

  Study of the mechanisms of assembly and budding of the arboviruses Oropouche and Zika in neurons and the effects of infection on neuronal proteostasis, AP.R

• Abstract

  Although dispensable for HIV replication in vitro, the Nef viral protein is critical for maintaining high viral loads in animal models and progression to AIDS in human patients. Nef interferes with the intracellular transport of host proteins involved in the virus replicative cycle and immunity. Among the targets of Nef is the SERINC5 protein, which, when incorporated into nascent viral p…

  Study of the molecular mechanisms used by the HIV-1 Nef protein to neutralize host cell defense strategies, AP.R

(Only some records are available in English at this moment)

• Abstract

  The Oropouche virus (OROV) is an emerging arbovirus associated with increasing outbreaks in Latin America and neurological manifestations, including encephalitis and meningitis. Despite its relevance, the molecular mechanisms underlying its neurovirulence remain poorly understood. Recent evidence from our group indicates that OROV infection may interfere with the homeostasis of the amyloi…

  Study on the role and homeostasis of amyloid precursor protein (APP) during Oropouche virus infection., BP.MS

• Abstract

  Oropouche virus (OROV), the etiological agent of Oropouche Fever, is an emerging arbovirus of major relevance in Latin America, responsible for epidemic outbreaks of acute febrile illness that may progress to severe forms associated with neurological complications, vertical transmission, and, in some cases, death. Originally restricted to the Amazon region, since 2023 OROV infections have…

  Investigation of the mechanisms of Oropouche virus entry and viral factory formation in human cells., BP.DD

• Abstract

  Unconventional protein secretion (UPS) is a cellular process that bypasses the classical endoplasmic reticulum-Golgi pathway and enables the direct release of proteins into the extracellular space. While the precise molecular details of UPS are still being elucidated, these mechanisms collectively enable the release of cytokines, growth factors, enzymes, and signaling molecules that play …

  Investigating GRASP Structure-Activity Relationship, Supramolecular Arrangements and Interaction Partners in Unconventional Secretion and Possible Implications for Orthobunyavirus Assembly., BP.PD

(Only some records are available in English at this moment)

• Abstract

  Para os estudos que compreendem o WP5 deste projeto temático será crucial a geração de importantes reagentes como: 1) vetores de expressão lentiviral codificando GRAPs e mutantes para experimentos de resgate de fenótipo descritos no Projeto, e 2) estoque do vírus Oropouche com titulo viral conhecido. Portanto, faz-se necessário uma pessoa com formação especial em nível superior e engajada…

  Production and testing of lentiviral vectors for expression of GRASP and mutant proteins in human cells, BP.TT

• Abstract

  The Oropouche virus (OROV) is an arbovirus of the Peribunyaviridae family and the Orthobunyavirus genus. Since its identification in the 1960s, it is estimated that at least half a million cases of OROV infection have occurred in Brazil. OROV is the causative agent of Oropouche fever, a febrile disease with symptoms similar to those of other tropical arboviruses and with the potential to …

  Study of the influence of Oropouche virus infection on the intracellular trafficking of amyloid precursor protein (APP) in mouse neurons., BP.IC

• Abstract

  The Zika virus (ZIKV) and the Oropouche virus (OROV) are emerging arboviruses of great importance to publichealth, and due to global climate changes, they have the potential to spread to new tropical and subtropicalregions. While ZIKV caused a severe epidemic in Brazil, OROV causes recurrent and recent outbreaks in theCaribbean and tropical regions of Latin America. Both viruses, despite …

  Study of the mechanisms of assembly and budding of the arboviruses Oropouche and Zika in neurons and the effects of infection on neuronal proteostasis., BP.JD

(Only some records are available in English at this moment)