Abstract
Adaptive Laboratory Evolution (ALE) is a powerful tool to shape microorganisms for industrial applications. This project aims at using ALE for optimizing Escherichia coli to growth under anaerobic conditions and to use sucrose as a carbon source. For these purposes, E. coli strains will be first engineered with the csc genes (cscB, cscK, cscA), for uptake of sucrose, and with the gene suc…