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Evolutionary engineering of Escherichia coli for anaerobic production of polyhydroxyalkanoates from sugarcane-derived sucrose

Grant number: 20/02246-0
Support Opportunities:Scholarships in Brazil - Doctorate (Direct)
Effective date (Start): June 01, 2020
Effective date (End): November 30, 2023
Field of knowledge:Biological Sciences - Microbiology - Applied Microbiology
Acordo de Cooperação: Netherlands Organisation for Scientific Research (NWO)
Principal Investigator:Jonas Contiero
Grantee:Joneclei Alves Barreto
Host Institution: Instituto de Pesquisa em Bioenergia (IPBEN). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil
Associated research grant:17/50249-6 - A novel strategy for anaerobic production of polyhydroxyalkanoates (PHA), AP.TEM


Adaptive Laboratory Evolution (ALE) is a powerful tool to shape microorganisms for industrial applications. This project aims at using ALE for optimizing Escherichia coli to growth under anaerobic conditions and to use sucrose as a carbon source. For these purposes, E. coli strains will be first engineered with the csc genes (cscB, cscK, cscA), for uptake of sucrose, and with the gene sucP, for sucrose phosphorolysis. Genetically modified strains will be then challenged (ALE approach) to propagate under anaerobic conditions and to consume increasing amounts of sucrose from synthetic medium. Mutations that accumulated during ALE will be discovered by next-generation sequencing of the genomes and then reverse engineered into the parental strains. The resulting strains will be further submitted to ALE for propagation in sugarcane-derived molasses and juices. Finally, strains that were optimized for anaerobic growth on sucrose will be subjected to Metabolic Engineering for polyhydroxyalkanoates (PHAs) synthesis, resulting in ideal strains for bioplastic production from sugarcane feedstock. (AU)

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